土壤2016，Vol.48Issue(5)：835-843,9.DOI:10.13758/j.cnki.tr.2016.05.001
土壤宏蛋白质组学之土壤蛋白质提取技术的发展
The Development of Soil Protein Extraction Methods in Soil Metaproteomics
摘要
Abstract
There has been increasing concern about the soil metaproteomics in recent years. Proteomics platform in soil is helpful for understanding the biogeochemical cycles of carbon, nitrogen and phosphorus and the oxidation of soil organic matter. By analyzing the sources, functions and activities of soil microbial protein, soil metaproteomics can address the issue of different kinds of protein expression and then link protein information with the associated ecosystem processes. However, although the species of protein in soils are very rich, the contents of proteins in soils are usually very low, which dramatically hinders the application of proteomics techniques. A critical step thus is to get a high concentration of protein solution by pretreatment of soil samples. This review summarized some typical methods for extracting proteins from soils and leaf litter in recent years. In addition, the potential directions of improvements for current methods were discussed.关键词
土壤蛋白质/宏蛋白质组学/蛋白质提取方法Key words
Soil protein/Proteomics/Protein extraction methods分类
农业科技引用本文复制引用
熊艺,林欣萌,兰平..土壤宏蛋白质组学之土壤蛋白质提取技术的发展[J].土壤,2016,48(5):835-843,9.基金项目
国家重点基础研究发展计划(973计划)项目(No.2015CB150501)和国家重点研发计划项目(2016YFD0200308)资助。 （973计划）
