西北师范大学学报（自然科学版）2016，Vol.52Issue(6)：85-91,102,8.DOI:10.16783/j.cnki.nwnuz.2016.06.016
白菜型油菜RAV基因的克隆及表达分析
Cloning and expression analysis of transcription factor RAV from Brasserie campestris
摘要
Abstract
The sequences of RAV gene from Brassica campestris is obtained by RACE method based on the RAV factor sequence of Arabidopsis thaliana ,the full-length cDNA of RAV is 1036 bp,containing a 5′-UTR of 109 bp,a 3′-UTR of 171 bp,and a 1026 bp opening reading frame.The deduced protein is 341 amino acids with molecular weight 38.03 u and isoelectric point 9.2.The transcription factor of RAV contains two distinct DNA domains,one AP2 domain together with one B3 domain.Comparison of amino acid sequences and phylogenetic analysis indicates that this gene has high identities with Arabidopsis thaliana (88.0%). qRT-PCR results indicates that RAV is induced by cold and NaCl. It shows the expression of the RAV plays an important role during abiotic stress in Brasserie campestris .关键词
油菜/RAV转录因子/克隆/表达分析Key words
Brassica campestris/RAV transcription factor/molecular cloning/expression analysis分类
农业科技引用本文复制引用
张腾国,李萍,寇明刚,王娟,郑晟..白菜型油菜RAV基因的克隆及表达分析[J].西北师范大学学报（自然科学版）,2016,52(6):85-91,102,8.基金项目
国家自然科学基金资助项目(31460099,31160089) （31460099,31160089）
