西北师范大学学报（自然科学版）2016，Vol.52Issue(6)：92-97,6.DOI:10.16783/j.cnki.nwnuz.2016.06.017
结合基因芯片和生物信息学工具构建D-半乳糖致衰老小鼠模型的分子调控网络
Analyzing the molecular regulatory networks in aging mouse induced by D-galactose using microarray combined with the MAS tool
摘要
Abstract
To explore the target genes related with aging and construct the molecular regulatory network in aging model,the aging mice induce by D-galactose are used.The genome-wide oligonucleotide microarray and MAS tool are used to analyze the network which the regulated genes are involved in aging model.The morphology of kidney of aging mice are abnormal adversely compared with normal mice.There are 232 significantly regulated genes (fold changes ≥ 2 )in aging mice verse normal mice. These differentially expresse genes participate in many important biological processes, including circadian rhythm, metabolism,and PPAR signaling pathway. There are four core genes in the network related with differentially expresse genes, including Arnt 1, Cidea , H spa 1b , and Anxa 13, which are potential targets of aging mechanism,and useful for drug screening.关键词
基因芯片/生物信息学/衰老模型/分子网络Key words
microarray/bioinformatics/aging model/molecular regulatory networks分类
医药卫生引用本文复制引用
王晶,赵翊,王勇,陈彻,李海龙..结合基因芯片和生物信息学工具构建D-半乳糖致衰老小鼠模型的分子调控网络[J].西北师范大学学报（自然科学版）,2016,52(6):92-97,6.基金项目
甘肃省高等学校科研项目(2013A-087) （2013A-087）
甘肃省财政厅项目(BH-2013-24) （BH-2013-24）
甘肃中医药大学中青年科研基金资助项目(ZQ2015-20) （ZQ2015-20）
