医药导报2016，Vol.35Issue(11)：1168-1172,5.DOI:10.3870/j.issn.1004-0781.2016.11.003
尿毒灵对转化生长因子-β1诱导的HK-2转分化的干预
Interference of Niaoduling Reverses TGF-β1-induced Epithelial-mesenchymal Transition in HK-2 Cells
摘要
Abstract
Objective To investigate the effect of Niaoduling on cell proliferation and morphological changes in the process of epithelial-mesenchymal transition(EMT)of renal tubular epithelial cells induced by TGF-β1 . Methods HK-2 cells were treated with various concentrations of TGF-β1 for 24,48 and 72 h,respectively. According to the cell proliferation and morphological changes,the best concentration and time points was selected to induce EMT.HK-2 cells were divided into nromal control group,model control group,treatment group in control condition and treatment group in model condition. The cell proliferation was detected by MTT and the cell morphological changes was observed by microscope. Results TGF-β1 inhibited cell proliferation and 5 ng·mL-1 for 72 h has the strongest effect(P<0.05).Under this condition,cells had the most obvious EMT-like appearance.Compared with the model control group,inhibition induced by TGF-β1 could be significantly reversed by Niaoduling at the dosage of 160 μg·mL-1(P<0.05).Morphological observation showed that Niaoduling could maintain normal cell morphology as paving stone,prevent fibrosis induced by TGF-β1 . Conclusion Niaoduling can partly reverse EMT induced by TGF-β1 in HK-2 cells.关键词
尿毒灵/转化生长因子 β1/人肾小管上皮细胞/转分化Key words
Niaoduling/TGF-β1/HK-2/Epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
党泽春,袁小红..尿毒灵对转化生长因子-β1诱导的HK-2转分化的干预[J].医药导报,2016,35(11):1168-1172,5.基金项目
广东省中医院中医药科学技术研究专项（ YN2014ZH07）；广东省教育厅科技创新项目（2012KJCX0035）；广东省科学技术厅产学研项目（2013B090700015）。 （）
