医药导报2016，Vol.35Issue(11)：1254-1258,5.DOI:10.3870/j.issn.1004-0781.2016.11.023
羟基喜树碱/GA-PEI-PLGA纳米粒的制备及其工艺优化
Preparation and Optimization of Hydroxycamptothecin Loaded GA-PEI-PLGA Nanoparticles
摘要
Abstract
Objective To prepare hydroxycamptothecin(HCPT)loaded glycyrrhetinic acid modified polyethyleneimine-poly(lactic-co-glycolic acid)( GA-PEI-PLGA)nanoparticles. Methods Emulsion-solvent evaporation method was used to prepare HCPT-loaded GA-PEI-PLGA nanoparticles. Based on the single-factor experiments,drug/ GA-PEI-PLGA ratio,oil phase/ water phase ratio,sonication timeand power were further investigated. The formulation and process were optimized by orthogonal testingdesign using entrapment efficiency and drug loading efficiency as indexes. HCPT-loaded GA-PEI-PLGA nanoparticles ˊ diameter and zeta potential were determined. Results HCPT-loaded GA-PEI-PLGA nanoparticles were successfully prepared by emulsion-solvent evaporation method.Drug/ GA-PEI-PLGA ratio,in particular,had the uniform particle on the preparation of HCPT-loaded nanoparticles.The optimal process was as follows:drug/ GA-PEI-PLGA ratio of 3 to10,oil phase/ water phase ratio of 1 to 9,sonication power of 570 W and sonication time of 15 min.The entrapment efficiency and drug loading efficiency of HCPT-loaded GA-PEI-PLGA nanoparticles were(87.52± 3.91)% and(20.10± 4.72)%,respectively. The average particle size was(218.1± 3.23)nm and the zeta potential was(34. 98 ± 3. 56)mV. Conclusion Emulsion-solvent evaporation method was feasible for preparing HCPT-loaded GA-PEI-PLGA nanoparticles,with uniform particel size distribution, good stability,high entrapment and drug loading efficiency.关键词
羟基喜树碱/GA-PEI-PLGA 纳米粒/正交设计实验/制备工艺Key words
Hydroxycampothecin GA-PEI-PLGA/Nanoparticles/Orthogonal design test/Preparation technology分类
化学化工引用本文复制引用
梁劲康,吴得天,胡巧红,吴雅琼..羟基喜树碱/GA-PEI-PLGA纳米粒的制备及其工艺优化[J].医药导报,2016,35(11):1254-1258,5.基金项目
广东药学院大学生创新创业训练项目 （）
