中国临床医学2016，Vol.23Issue(4)：428-432,5.DOI:10.12025/j.issn.1008-6358.2016.20160671
胰腺癌细胞纳米探针的磁共振荧光双模态成像及抗肿瘤效果观察
Effects of QDs@ Gd-RGD nano probe on MRI and fluorescent dual-modality imaging of pancreatic cancer cells and its anti-tumor role
摘要
Abstract
Objective:To investigate the effects of QDs@ Gd‐RGD nano probe on fluorescent and MRI dual‐modality imaging of M IA Ⅱ pancreatic cancer cells and its role in inhibiting pancreatic cancer cells .Methods:QDs@ Gd‐RGD nano probe was prepared .QDs@ Gd‐RGD nano probe (QDs@ Gd‐RGD group) and Gd (Gd group) were dissolved in phosphate buffer solution (PBS) and incubated with MIA Ⅱ pancreatic cancer cells for 1 h ,and no treatment was given to the control group .And then imaging characteristics of the 3 groups were observed by fluorescence phase contrast microscope and 1 .5 T MRI observation .After 3 groups of MIA Ⅱ pancreatic cancer cells were cultured for 24 h ,cell morphology ,proliferation , cloning ,migration ,cycle and apoptosis of MIA Ⅱ pancreatic cancer cells were observed .Results:Fluorescence phase contrast microscopy showed that the QDs@ Gd‐RGD nanoparticles were distributed in MIA Ⅱ pancreatic cancer cell membrane ,while no cell membrane and cell fluorescence distribution was observed in the Gd group and the control group .1 .5 T MRI imaging showed that the signal intensity of QDs@ Gd‐RGD group was significantly higher than that of the Gd group and the control group (P<0 .05) .QDs@ Gd‐RGD group MIA Ⅱ pancreatic cancer cells lost the typical paving‐stone morphology ,nuclear fragmentation and cytoplasmic vacuolization took place , cell cloning ability decreased significantly , cell proliferation was inhibited ,cell migration ability decreased ,cell cycle was significantly blocked and cell apoptosis increased (P< 0 .05) . Conclusions:QDs@ Gd‐RGD nano probe is effective in fluorescence and MRI dual modality imaging of MIA Ⅱ pancreatic cancer cells .Meanwhile ,QDs@ Gd‐RGD nano probe has a certain killing effect on MIA Ⅱ pancreatic cancer cells .关键词
胰腺癌细胞/QDs Gd-RGD/M RI荧光双模态成像/靶向诊断/抗肿瘤治疗Key words
pancreatic cancer cell/QDs Gd-RGD/MRI and fluorescent dual-modality imaging/targeted diagnosis/anti-tumor therapy分类
医药卫生引用本文复制引用
刘峰君,叶雯,何欣源,施裕新..胰腺癌细胞纳米探针的磁共振荧光双模态成像及抗肿瘤效果观察[J].中国临床医学,2016,23(4):428-432,5.基金项目
上海市科学技术委员会上海市自然基金青年项目（13ZR1459400）．Supported by the Program of Science and Technology Commission of Shanghai Municipal for Young Scientists （13ZR1459400）． （）
