中国药房2016，Vol.27Issue(31)：4357-4359,3.DOI:10.6039/j.issn.1001-0408.2016.31.11
黄酮哌酯对前列腺增生模型大鼠的保护作用机制研究
Study on the Protective Mechanism of Flavoxate on Prostatic Hyperplasia in Model Rats
摘要
Abstract
OBJECTIVE:To study the protective mechanism of flavoxate on prostatic hyperplasia in model rats. METHODS:The rats were randomly divided into sham operation group,model group and flavoxate high-dose and low-dose groups (80,40 mg/kg),with 8 rats in each group. Except for sham operation group,those groups were emasculated and given sterandryl to induce BPH model. They were given relevant medicine intragastrically 1 h after administration of sterandryl,once a day,for consecutive 4 weeks. Sirius red and Masson staining were used to detect the precipitation of collagen in prostate;immunohistochemistry was used to detect the expressions of prostatic type Ⅰ collagen,FN and E-cadherin;ELISA assay was used to determine the levels of SOD, GPx,MDA,EGF and VEGF in prostate. qPCR assay was used to detect the levels of miR-21 and TGF-β1. RESULTS:Compared with the sham operation group,the prostatic collagen was accumulated;prostatic index,the expression of FN,MDA content and levels of VEGF,EGF,miR-21 and TGF-β1 were increased(P<0.05);the expression of E-cadherin,and the activity of SOD and GPx were decreased (P<0.05). Compared with model group,the precipitation of prostatic collagen was decreased;prostatic in-dex,the expression of FN,MDA content,the levels of VEGF,EGF,miR-21 and TGF-β1 were decreased in flavoxate high-dose and low-dose groups (P<0.05);while the expression of E-cadherin and the activity of SOD and GPx were increased (P<0.05). CONCLUSIONS:Flavoxate can inhibit BPH,the mechanism of which may associated with down-regulating the expressions of miR-21 and TGF-β1,inhibiting oxidative stress and angiogenesis and improving epithelial-mesenchymal transition.关键词
黄酮哌酯/前列腺增生/氧化应激/血管生成/上皮-间质转化/大鼠Key words
Flavoxate/Benign prostatic hyperplasia/Oxidative stress/Angiogenesis/Epithelial-mesenchymal transition/Rats分类
医药卫生引用本文复制引用
杜丽芬,陈镜楼..黄酮哌酯对前列腺增生模型大鼠的保护作用机制研究[J].中国药房,2016,27(31):4357-4359,3.基金项目
湖北省自然科学基金资助项目（No.2015CFB250）；武汉市中青年医学骨干人才培养工程 （）
