中国药房2016，Vol.27Issue(31)：4363-4366,4.DOI:10.6039/j.issn.1001-0408.2016.31.13
云芝多糖对小鼠黑色素瘤B16细胞体外增殖和凋亡的影响及其机制Δ
Effects of Coriolus versicolor Polysaccharides on Proliferation and Apoptosis of Murine Melanoma B16 Cell in vitro and Its Mechanism of Action
摘要
Abstract
OBJECTIVE:To study the effects of Coriolus versicolor polysaccharides(CVP)on in vitro proliferation and apop-tosis of murine melanoma B16 cell and its mechanism. METHODS:MTT assay was used to determine the survival rate of murine melanoma B16 cell after cultured with DMSO (negative control),0 (blank control),0.156,0.312 5,0.625,1.25,2.5,5.0 and 10.0μg/ml CVP for 48,72 h,and the IC50 was calculated. Flow cytometry was used to determine the apoptotic rate of murine mela-noma B16 cell after cultured with DMSO(negative control),0(blank control),0.5μg/ml CVP for 24,48,72 h. Quantitation Re-al-time PCR(qRT-PCR)was used to determine the mRNA expression of cell apoptosis-related gene P53,Bcl-2 and Fas in murine melanoma B16 cell after cultured with DMSO(negative control),0.5 μg/ml CVP for 48,72 h. RESULTS:0.156-10.0 μg/ml CVP could inhibit the growth of B16 cells,in concentration and time-dependent manner within 0.156-0.625 μg/ml;IC50 of B16 cells af-ter cultured for 48 and 72 h were(0.32±0.01),(0.18±0.04)µg/ml. After cultured with 0.5μg/ml CVP for 24,48,72 h,apoptot-ic rates of B16 cells were increased significantly,compared to negative control and blank control (P<0.01). After cultured with 0.5 μg/ml CVP for 48,72 h,mRNA expression of P53,Bcl-2 and Fas were decreased significantly,compared to negative control (P<0.01). CONCLUSIONS:CVP can inhibit the proliferation and induce the apoptosis of B16 cells,the mechanism of which may be associated with the expression down-regulation of P53,Bcl-2 and Fas gene.关键词
云芝多糖/黑色素瘤B16细胞/增殖/凋亡/体外Key words
Coriolus versicolor polysaccharides/Murine melanoma B16 cell/Proliferation/Apoptosis/in vitro分类
医药卫生引用本文复制引用
魏士杰,陈文强..云芝多糖对小鼠黑色素瘤B16细胞体外增殖和凋亡的影响及其机制Δ[J].中国药房,2016,27(31):4363-4366,4.基金项目
陕西省“13115”科技创新工程计划项目 （）
