中国药房2016，Vol.27Issue(31)：4370-4372,3.DOI:10.6039/j.issn.1001-0408.2016.31.15
菝葜乙酸乙酯部位对H22荷瘤小鼠的抑瘤作用及其机制研究
Anti-tumor Effect of Ethyl Acetate Part of Smilaz china on H22 Liver Cancer-bearing Mice and Its Mecha-nism Study
摘要
Abstract
OBJECTIVE:To study the anti-tumor effects of ethyl acetate part of Smilaz china on H22 liver cancer-bearing mice and its mechanism. METHODS:90 mice were randomly divided into normal group,model group,positive control group (cytox-an,0.02 g/kg,ip,every 2 days)and ethyl acetate part of S. china low-dose,medium-dose and high-dose groups [1.0,2.0,4.0 g (crude drug)/kg,ig,twice a day]. Except for normal group,H22 transplanted models of mice were established. The next day after modelation,treatment groups were given relevant medicine,and normal group and model group were given constant volume of 0.5% sodium carboxymethylcellulose solution intragastrically. The change of body weight and tumor weight were observed after medication;the serum levels of VEGF and neoangiogenesis(NOS)were determined as well as vascular permeability of tumor;the expression of CD31 was detected in tumor tissue. RESULTS:Compared with model group,body weight and tumor weight of posi-tive control group were decreased significantly,while the serum levels of VEGF and NOS,vascular permeability of tumor were in-creased significantly(P<0.05 or P<0.01). Tumor weight and the expression of CD31 were decreased significantly in ethyl acetate part of S. china medium-dose and high-dose groups,while serum levels of VEGF and NOS,vascular permeability of tumor were in-creased significantly (P<0.05 or P<0.01). Tumor weight were decreased significantly in ethyl acetate part of S. china low-dose group,while vascular permeability of tumor was strengthened(P<0.05 or P<0.01). CONCLUSIONS:The ethyl acetate part of S. china shows anti-tumor effects in H22 liver cancer-bearing mice,and its mechanism might be associated with reducing the serum lev-els of VEGF and NOS,improving vascular permeability of tumor and inhibiting neovascularization.关键词
菝葜/乙酸乙酯部位/抑瘤作用/H22荷瘤小鼠/血管内皮生长因子/一氧化氮合酶Key words
Smilax china/Ethyl acetate part/Anti-tumor effect/H22 liver cancer-bearing mice/VEGF/NOS分类
医药卫生引用本文复制引用
吴先闯,刘瑜新,宋卫中,宋晓勇,康文艺,侯彦涛,张永州,张晓坚,张凌云,卢红..菝葜乙酸乙酯部位对H22荷瘤小鼠的抑瘤作用及其机制研究[J].中国药房,2016,27(31):4370-4372,3.基金项目
河南省科技发展计划项目 （）
