中国药房2016，Vol.27Issue(31)：4403-4405,4406,4.DOI:10.6039/j.issn.1001-0408.2016.31.25
载纳豆激酶的三甲基壳聚糖纳米粒的制备及体外释放度研究Δ
Preparation of Nattokinase-loaded Trimethyl Chitosan Nanoparticles and Study on Its Release Rate in vitro
摘要
Abstract
OBJECTIVE:To prepare nattokinase (NK)-loaded trimethyl chitosan (TMC) nanoparticles (TMC-NK-NPs),and to study its release rate in vitro. METHODS:TMC with different degree of quaternization(15%,20%,25%)were synthetized,i.e. TMC15,TMC20,TMC25;those were combined with NK to form TMC-NK-NPs by self-assembly. The particle size,polydispersi-ty index (PDI) and Zeta potential were determined and TCM was screened;the morphology, entrapment efficiency (EE), drug-loading amount,30 d stability at 4 ℃ and dark place,24 h accumulative release rate in vitro (Q) and NK activity of TMC-NK-NPs by optimal formulation were observed and determined. RESULTS:Using TMC20,TMC-NK-NPs were smaller in particle size [(161.0 ± 4.8) nm],the lowest in PDI (0.204);the Zate potential was (19.2 ± 1.5) mV,spherical or spherical-like, EE was(45.4±1.51)%,drug-loading amount was(14.2±0.25)%,stable within 30 d,the peak Q12 h was 92.3%. The maximum released activity of NK was 76.6% at 1 h,but then gradually decreased with time. CONCLUSIONS:TMC-NK-NPs are prepared successfully,round and complete with high EE and drug-loading amount,good sustained-release property.关键词
三甲基壳聚糖/纳豆激酶/纳米粒/体外释放度/制备Key words
Trimethyl chitosan/Nattokinase/Nanoparticles/Release rate in vitro/Preparation分类
医药卫生引用本文复制引用
廖杰,任晓婷,尹宗宁..载纳豆激酶的三甲基壳聚糖纳米粒的制备及体外释放度研究Δ[J].中国药房,2016,27(31):4403-4405,4406,4.基金项目
国家自然科学基金资助项目 （）
