中国药房2016，Vol.27Issue(31)：4406-4410,5.DOI:10.6039/j.issn.1001-0408.2016.31.26
雪松松针总皂苷的超声提取工艺优选及体外抗肿瘤活性研究
Optimization of the Ultrasonic Extraction Technology of Total Saponins from Pine Needle of Cedrus deoda-ra and Study on Its in vitro Antitumor Activity
摘要
Abstract
OBJECTIVE:To optimize the ultrasonic extraction technology of total saponins from pine needles of Cedrus deoda-ra(TSPNCD),and to investigate its in vitro antitumor activity. METHODS:The content of TSPNCD was determined by UV spec-trophotometry. Based on single factor test,ethanol volume fraction,solvent-material ratio and extraction time were selected as influ-ential factors during ultrasonic extraction. According to Box-Behnken design,using the content of TSPNCD as response value,re-sponse surface analysis was conducted and used to optimize the ultrasonic extraction technology of TSPNCD. The effects of TSPNCD on the proliferation of human lung cancer A549 cells,human hepatocellular carcinoma HepG2 cells and human gastric cancer MKN45 cells were detected,and IC50 of them were calculated. RESULTS:Optimal technology was that extraction solvent was 73% ethanol;solvent-material ratio was 13∶1;ultrasonic extraction times was twice,each time for 65 min. Under the above conditions,the average content of TSPNCD reached 6.693 mg/g,and relative error between it and predicted value (6.508 mg/g) was 2.84%. TSPNCD inhibited the growth of A549 cells,HepG2 cells and MKN45 cells in a dose-dependent manner,with the IC50 values of (63.98 ± 6.79),(154.91 ± 10.20),(176.32 ± 14.26) µg/ml,respectively. CONCLUSIONS:The ultrasonic extraction technology was verified as reasonable and feasible. TSPNCD has certain inhibitory effect on the proliferation of A549 cells,HepG2 cells and MKN45 cells,especially for A549 cells.关键词
雪松松针/总皂苷/超声提取工艺/Box-Behnken响应面法/抗肿瘤活性/体外Key words
Pine needles of Cedrus deodara/Total saponins/Ultrasonic extraction technology/Box-Behnken response surface methodology/Antitumor activity/in vitro分类
医药卫生引用本文复制引用
李师,雷艳萍,石晓峰,刘东彦,王斌利,张莉霞..雪松松针总皂苷的超声提取工艺优选及体外抗肿瘤活性研究[J].中国药房,2016,27(31):4406-4410,5.基金项目
甘肃省科技支撑计划项目（No.甘财教〔2012〕197号）；兰州市人才创新创业项目（No.2014-RC-62）；甘肃省高校中（藏）药化学与质量研究省级重点实验室开放基金项目 （）
