中国药房2016，Vol.27Issue(31)：4421-4424,4.DOI:10.6039/j.issn.1001-0408.2016.31.30
黔产毛蒟挥发油的提取工艺优化及化学成分分析
Optimization of the Extraction Technology of Volatile Oil from Guizhou Piper puberulum and Analysis of Its Chemical Compositions
摘要
Abstract
OBJECTIVE:To optimize the extraction technology of volatile oil from Guizhou Piper puberulum and analyze its chemical compositions. METHODS:The volatile oil was extracted from Guizhou P. puberulum by steam distillation(HD method). Orthogonal test was designed using the extract rate of volatile oil as index,with soaking time,extraction time,material-liquid ratio (g/ml) and smashed particle size as factors. Validation test was also conducted. GC-MS was adopted to separate and identify the chemical compositions of the volatile oil from Guizhou P. puberulum,and area normalization method was used for the determina-tion of relative percentage of each composition. RESULTS:The optimal extraction technology was as follows as soaking time of 3 h,extraction time of 5 h,material-liquid ratio of 1∶10 and degree of grinding 12 meshes;in validation test,average extract rate of volatile oil was 59.83%(RSD=0.905 3%,n=3). 78 compounds were identified,and relative percentage was 81.125%;there were four main compositions such as γ-elemene(11.858%),4-vinyl-α,4-trimethyl-3-(1-methylvinyl)-cyclohexane methanol(8.701%), carnation(8.443%)and α-caryophyllene(6.216%). CONCLUSIONS:Optimized HD method is simple in production equipment, low in production cost,good in repeatability;determination method of validation oil is simple and feasible,and can analyze chemi-cal compositions of volatile oil from Guizhou P. puberulum.关键词
黔产毛蒟/挥发油/水蒸气蒸馏法/气相色谱-质谱法/成分Key words
Guizhou Piper puberulum/Volatile oil/Steam distillation method/GC-MS/Compositions分类
医药卫生引用本文复制引用
杨艳,韦余,王玉和,鄢乾燕..黔产毛蒟挥发油的提取工艺优化及化学成分分析[J].中国药房,2016,27(31):4421-4424,4.基金项目
贵州省科学技术基金项目 （）
