中国药房2016，Vol.27Issue(31)：4429-4432,4.DOI:10.6039/j.issn.1001-0408.2016.31.32
大高良姜不同部位总黄酮的提取工艺优化及体外抗氧化活性研究
Optimization of the Extraction Technology and Antioxidant Activity Study in vitro of Total Flavones from Different Parts of Alpinia galangal
摘要
Abstract
OBJECTIVE:To optimize the extraction technology of total flavonoids from the root,stem and leaves of Alpinia galangal and compare the antioxidant activities of the flavonoids in vitro. METHODS:Taking into account the four factors,such as the ratio of solid to liquid,the volume fraction of ethanol,the temperature of enzymatic hydrolysis and the time of ultrasonic,us-ing the extraction rate of total flavonoids as index,with the method of ultrasonic assisted,the extraction technology of total flavo-noids from A. galangal was optimized by Box-Behnken response surface test on the basis of single factor experiment. The antioxi-dant activity tests in vitro of different concentrations of total flavonoids (0.1-1.0 mg/ml) from different parts of A. galangal were conducted,including DPPH free radical clearance test,O2- free radical clearance test,protective test of vegetable and animal fat [using vitamin C(VC)as control]. RESULTS:The optimal extraction technology as follows as the ratio of solid to liquid was 1∶23.8;the volume fraction of ethanol was 60%;water bath temperature was 80 ℃;ultrasonic time was 33.5 min. Under this condi-tion,the extraction rates of total flavonoids from the root,stem and leaves of A. galangal were 53.5,52.7,52.5 mg/g. The rates of their relative error from predicted value were all no more than 2.48%. When the mass concentration was 0.5 mg/ml,the antioxidant capacity of total flavonoids from different parts of A. galangal to DPPH free radicals was as follows as leaves (94.3%)>root (93.6%)>stem(90.8%);the antioxidant capacity of VC was 96.4%. When the mass concentration was 1.0 mg/ml,the antioxi-dant capacity to O2- free radicals was as follows as root(63.7%)>stem(60.2%)>leaves(42.9%);the antioxidant capacity of VC was 94.7%. When the mass concentration was 0.4 mg/ml,the protective rate to vegetable fat was as follows as leaves (39.7%)>stem(30.6%)>root(28.3%);the protective rate to animal fat was as follows as leaves(40.6%)>stem(30.2%)>root(29.3%);the protective rate of VC was about 60%. CONCLUSIONS:Optimized extraction technology is feasible,and can be used for the extraction of total flavones from different parts of A. galangal. Total flavones from different parts have certain antiox-idant activity,specially the total flavones from leaves of A. galangal is stronger than other.关键词
大高良姜/根/茎/叶/总黄酮/提取工艺/Box-Behnken响应面法/抗氧化活性Key words
Alpinia galangal/Root/Stem/Leaves/Total flavones/Extraction technology/Box-Behnken response surface methodology/Antioxidant activity分类
医药卫生引用本文复制引用
刘源,唐斌,张孝琴,杨刚..大高良姜不同部位总黄酮的提取工艺优化及体外抗氧化活性研究[J].中国药房,2016,27(31):4429-4432,4.基金项目
2014年泸州市科技局项目[No.2014（56）]；2015年西南医科大学科研基金 （）
