中国药理学通报2016，Vol.32Issue(11)：1516-1520,5.DOI:10.3969/j.issn.1001-1978.2016.11.008
1-磷酸鞘氨醇促进胰岛素分泌及可能机制研究
Effects and possible mechanism of sphingosine-1-phosphate-stimulated insulin secretion from rat islets
摘要
Abstract
Aim To observe the effects of glucose-stim-ulated insulin secretion ( GSIS ) on rat islets after S1 P treatments and the underlying molecular mechanisms. Methods Collagenase P and Histopaque 1077 were used to digest and isolate rat pancreatic islets, and Dispase II was used to digest pancreatic islet to obtain pancreatic cells. Insulin secretions were measured after S1P (0~20 μmol·L-1 ) treatment under low glucose ( LG, 2. 8 mmol·L-1 ) and high glucose ( HG, 16. 7 mmol·L-1 ) conditions. Patch-clamp recordings were applied to monitor voltage-dependent potassium chan-nel currents (Kv currents) after S1P treatment. Calci-um image technique was used to measure the changes of intracellular Ca2+ concentration after S1P ( 0 ~20μmol·L-1 ) treatments. Results HG group signifi-cantly increased insulin secretion compared to LG group ( P<0. 01 ) . S1 P had no effect on insulin se-cretion under LG condition ( P>0. 05 ) . S1 P increased insulin secretion significantly in a dose-dependent man-ner under HG condition ( P<0. 01 ) . Kv currents ofβcells were inhibited significantly after S1 P treatment ( P<0. 01 ) . S1 P increased the concentrations of in-tracellular Ca2+ in a dose-dependent manner under HG condition( P <0. 01) . Conclusion S1P may pro-mote GSIS by inhibiting Kv currents and increasing the level of intracellular Ca2+.关键词
1-磷酸鞘氨醇/Ca2+/胰岛素分泌/电压依赖性钾通道/SD大鼠/β细胞Key words
sphingosine-1-phosphate/Ca2+/insulin secretion/voltage-dependent potassium channels/SD rat/β cell分类
医药卫生引用本文复制引用
赵艳丽,丁亚琴,王慧,章毅,杨静,刘云峰..1-磷酸鞘氨醇促进胰岛素分泌及可能机制研究[J].中国药理学通报,2016,32(11):1516-1520,5.基金项目
国家自然科学基金资助项目(No 81273564,81373464) （No 81273564,81373464）
山西省自然科学基金资助项目(No 201401143) （No 201401143）
山西省回国留学人员科研资助项目( No 2013-111) （ No 2013-111）
中华医学会临床医学科研专项资金项目(No 13040440429) （No 13040440429）
山西医科大学第一医院青年创新基金资助项目( No YC1422) （ No YC1422）
山西省留学回国人员科技活动项目择优资助项目(No 2016-97) （No 2016-97）
