中国药理学通报2016，Vol.32Issue(11)：1526-1530,5.DOI:10.3969/j.issn.1001-1978.2016.11.010
姜黄素烟酸酯对血管平滑肌细胞增殖及ERK1/2信号通路的影响
Effects of CurTn on proliferation of VSMC
摘要
Abstract
Aim To explore the anti-proliferation effects of curcumin trinicotinate ( CurTn ) on vascular smooth muscle cell ( VSMC ) and its mechanism. Methods The cells were cultured in DMEM supple-mented with 10% fetal bovine serum. MTT assay was used to examine cell proliferation. FCM was used to observe cell cycle. The expressions of PCNA, Cy-clinD1 and p-ERK1/2 were analyzed using Western blot. Results CurTn could inhibit the proliferation of VSMC and showed a certain amount-time relationship. What’ s more, CurTn could increase the G1 phase pro-portion of cell, decrease the S phase proportion and the expression level of PCNA protein. It was also found that CurTn significantly inhibit the protein expression of p-ERK1/2 and Cyclin D1 . Conclusion CurTn may inhibit the proliferation of VSMC via downregulating the expression of CyclinD1 and p-ERK1/2 .关键词
姜黄素烟酸酯/烟酸/姜黄素/VSMC/增殖/ERK/机制Key words
curcumin trinicotinate/niacin/curcu-min/VSMC/proliferation/ERK/mechanism分类
医药卫生引用本文复制引用
杨冬梅,阳巍,邱飞,孙四玉,陈剑雄,张彩平,熊国祚,庹勤慧,廖端芳..姜黄素烟酸酯对血管平滑肌细胞增殖及ERK1/2信号通路的影响[J].中国药理学通报,2016,32(11):1526-1530,5.基金项目
国家自然科学基金资助项目( No 81673722,816700291,81670268) （ No 81673722,816700291,81670268）
湖南省自然科学基金资助项目( No 14JJ1024,2015JJ2117) （ No 14JJ1024,2015JJ2117）
湖南省教育厅重点项目(No 16A156) （No 16A156）
