中国药理学通报2016，Vol.32Issue(11)：1531-1538,1539,9.DOI:10.3969/j.issn.1001-1978.2016.11.011
胞外酸化对大鼠关节软骨细胞焦亡的影响及可能机制
Effects of extracellular acidosis on pyroptosis of rat articular chondrocytes and its possible mechanisms
摘要
Abstract
Aim To study the effects of extracellular acidosis on articular chondrocytes pyroptosis and its possible mechanisms. Methods Primary articular chondrocytes were incubated in different pH and NAC. The expression of proinflammatory cytokines IL-1β, IL-18, ASC, NLRP3, caspase-1 were detected by Western blot and real-time PCR. The state of pyropto-sis was identified by AO/EB staining and LDH con-tents. The expression of ROS was observed by DCFH-DA, and ELISA was used to detect the IL-1β,IL-18 in cultured supernatants. Results Compared with the normal cell, extracellular acidosis could increase the expression of IL-1β, IL-18, ASC, NLRP3 and caspase-1 , upregulate the fluorescence intensity of in-tercellular ROS, accompanied with the promoted release of LDH. Moreover, it is observed that extra-cellular acidosis could also induce chondrocytes death by AO/EB staining. NAC,the scavenger of ROS could inhibit these effects of extracellular acidosis on chon-drocytes. Conclusion Extracellular acidosis may in-duce chondrocyte pyroptosis via upregulating the intra-cellular ROS content.关键词
关节炎/关节软骨细胞/细胞焦亡/炎症小体/ROS/NACKey words
arthritis/articular chondrocytes/pyropto-sis/inflammasome/ROS/NAC分类
医药卫生引用本文复制引用
吴小山,陈飞虎,葛金芳,周仁鹏,祖胜芹,朱传君..胞外酸化对大鼠关节软骨细胞焦亡的影响及可能机制[J].中国药理学通报,2016,32(11):1531-1538,1539,9.基金项目
国家自然科学基金资助项目(No 81271949,30873080) （No 81271949,30873080）
