中国药理学通报2016，Vol.32Issue(11)：1539-1542,4.DOI:10.3969/j.issn.1001-1978.2016.11.012
甲基莲心碱体外抑制人肝癌细胞增殖和侵袭的作用机制研究
Effect of neferine on proliferation and invasion of human hepatocellular carcinoma cell line HepG2 and Bel-7402
摘要
Abstract
Aim To investigate the effect of neferine on proliferation and invasion of human hepatocellular car-cinoma. Methods HepG2 and Bel-7402 cells were cultured in vitro with different concentrations of nefer-ine, then cell proliferation was observed by CCK-8 as-say; cell invasion was observed by transwell invasion assay; the protein expression of RhoA, RhoC and ROCK was detected by Western blot. Results CCK-8 results showed that neferine could significantly inhibit cell proliferation in a dose-dependent manner compared with the control group. Transwell invasion assay showed that cell invasion was significantly decreased with neferine 3 μmol · L-1 . Western blot results showed that RhoA, RhoC and ROCK protein expres-sion was decreased when neferine was co-incubated with hepatocellular carcinoma cells. Conclusion Nef-erine can inhibit proliferation and invasion of HepG2 and Bel-7402 cells, which is mediated mainly by the inhibition of RhoA, RhoC and ROCK protein expres-sion.关键词
甲基莲心碱/人肝癌细胞 HepG2/人肝癌细胞Bel-7402/增殖/侵袭/RhoA/RhoC/ROCKKey words
neferine/human hepatocellular carcinoma cell HepG2/human hepatocellular carcinoma cell Bel-7402/proliferation/invasion/RhoA/RhoC/ROCK分类
医药卫生引用本文复制引用
周雅君,时菁,田耕,周汉新,高国全..甲基莲心碱体外抑制人肝癌细胞增殖和侵袭的作用机制研究[J].中国药理学通报,2016,32(11):1539-1542,4.基金项目
深圳市科技计划项目( No JCYJ20140414170821259) （ No JCYJ20140414170821259）
深圳细胞生物技术转化医学重点实验室项目( No ZDSY20130531165409949) （ No ZDSY20130531165409949）
