中国药理学通报2016，Vol.32Issue(11)：1543-1547,1548,6.DOI:10.3969/j.issn.1001-1978.2016.11.013
何首乌中大黄素对L02肝细胞CYP亚酶表达及细胞毒性的影响
Effects of emodin in Polygonum multiflorum on liver cytotoxicity and CYP450 isoenzymes expression in L02 cells
摘要
Abstract
Aim To study the effect of emodin in Po-lygonum multiflorum on the expression of CYP450 isoenzymes in L02 hepatocytes and explore its mecha-nism of cytotoxicity. Methods L02 cells were treated with different concentrations of emodin. Cell viability was examined by MTS assay kit, and cell membrane injury was examined by detecting the release rate of lactate dehydrogenase( LDH) . The expression of cyto-chrome P450 mRNA was detected by real time PCR. Results The result of MTS assay showed that L02 cells viability was significantly reduced following expo-sure to emodin in a concentration and time dependent manner. The LDH release rate of L02 cells significant-ly increased after exposure to emodin for 48 h com-pared with the control group. On the mRNA level, compared with the control group,emodin had inductive effects on mRNA of each CYP450 enzyme, while had significant inductive effects on mRNA of CYP1 A1 and CYP1 B1 in a concentration and time dependent man-ner. Conclusion Emodin in Polygonum multiflorum may generate significant liver injury in L02 cells and has inductive effects on CYP450 enzyme activity.关键词
何首乌/大黄素/CYP450/肝损伤/L02肝细胞/ABT/mRNA表达Key words
Polygonum multiflorum Thunb./emodin/CYP450/liver injury/L02 cells/ABT/mRNA expres-sion分类
医药卫生引用本文复制引用
汪美汐,王宇光,徐焕华,张照研,马增春,肖成荣,谭洪玲,汤响林,高月..何首乌中大黄素对L02肝细胞CYP亚酶表达及细胞毒性的影响[J].中国药理学通报,2016,32(11):1543-1547,1548,6.基金项目
北京市自然科学基金资助项目(No 7164291) （No 7164291）
国家科技重大专项(No 2015ZX09501004-003-003) （No 2015ZX09501004-003-003）
国家重点基础研究发展计划(973计划)资助项目( No 2011CB505300,2011CB505304) （973计划）
