中国药理学通报2016，Vol.32Issue(11)：1553-1558,6.DOI:10.3969/j.issn.1001-1978.2016.11.015
基于高迁移率族蛋白B1对老鼠生物碱A抗肝纤维化作用机制的研究
Anti-hepatic fibrotic mechanism of Acanthus ilicifolius alkaloid A involved in high mobility group box 1
摘要
Abstract
Aim To investigate anti-hepatic fibrotic mechanism of Acanthus ilicifolius alkaloid A ( HBOA ) involved in high mobility group box 1 ( HMGB1 ) . Methods A hepatic fibrosis model of rat was estab-lished by the olive oil of CCl4 for 12 weeks. Then, at the 8th week,the successful model rats were randomly divided into model control group, colchicine group, HBOA high-dose group and HBOA low-dose group. From the 9th week,the rats in each group were treated with the drugs daily for 4 weeks respectively. The changes of liver histopathology and collagen were ob-served by HE staining and Masson staining, and the serum indicators including aspartate aminotransferase (AST),alanine aminotransferase(ALT) , total biliru-bin ( T-BIL ) , HMGB1 , interleukin-1β( IL-1β) and tumor necrosis factor-α( TNF-α) were determined. Moreover , the protein of HMGB1 in liver was examined by immunohistochemistry, and the expression of HMGB1 mRNA was measured by real-time fluores-cence quantitative PCR. Results Compared with the model control group,HBOA high-dose and HBOA low-dose groups significantly attenuated the fibrotic degree induced by CCl4 , markedly decreased the levels of ALT, AST, T-BIL, HMGB1, IL-1β, TNF-α. Moreo-ver, the expression of HMGB1 protein and mRNA in liver was decreased. And furthermore, serum HMGB1 level had significant positive correlation with IL-1β, TNF-α,ALT,AST and T-BIL. Conclusion HBOA has beneficial effects against liver fibrosis in rat which is induced by CCl4 , the mechanisms may be related to the inhibition of inflammatory response to HMGB1 .关键词
高迁移率族蛋白B1/老鼠簕生物碱A/肝纤维化/CCl4/炎症因子/大鼠Key words
high mobility group box 1/Acanthus ilicifo-lius alkaloid A/hepatic fibrosis/CCl4/inflammatory factor/rat分类
医药卫生引用本文复制引用
莫思燕,韦明中,邱金慧,朱勋帅,刘林,林军..基于高迁移率族蛋白B1对老鼠生物碱A抗肝纤维化作用机制的研究[J].中国药理学通报,2016,32(11):1553-1558,6.基金项目
广西高校科学技术研究项目(No 201106LX095) （No 201106LX095）
广西省自然科学基金资助项目(No 2013YB054) （No 2013YB054）
