中国药理学通报2016，Vol.32Issue(11)：1559-1564,1565,7.DOI:10.3969/j.issn.1001-1978.2016.11.016
参麦方对于大鼠心肌损伤保护作用的代谢组学研究
A metabolomics study on Shen-Mai decoction’ s protection of myocardial injured rats
摘要
Abstract
Aim With metabolomics method, to study Shen-Mai decoction’ s function on protecting the myo-cardial injured rats caused by doxorubicin for probing into the functioning mechanism of Shen-Mai decoction’ s medical effect. Methods By means of UPLC-TOF-MS, the metabolites of urine of the rats treated by Shen-Mai decoction were analyzed. Then, the differ-ences between each group of the metabolites were sought with PLS-DA ( the partial least square discrimi-nant analysis ) and OPLS-DA ( the orthogonal partial least squares discriminant analysis ) . VIP ( variable importance in projection ) and t test were used to screen out potential biomarkers. Results Fourteen endogenous metabolites such as succinyladenosine, a-denosine 2′, 3′-cyclic phosphate, S-( 3-methylbu-tanoyl )-dihydrolipoamide-E, cis-4-hydroxycyclohexy-lacetic acid, phenylbutyrylglutamine, 3-butyn-1-al, 3-hydroxytetradecanedioic acid, dihydrolipoamide and pyruvic acid, etc. were characterized. Conclusions The results indicate that Shen-Mai decoction can pro-tect the body from myocardial injury by regulating pu-rine metabolism, some acid metabolism, fat metabo-lism and energy metabolism, etc. The study expounds the functioning mechanism for Shen-Mai decoction ’ s medical effect in the body and provides theoretical grounds for the rationality of the two medical herbs ’ compatibility and their combination in clinical treat-ment of diseases.关键词
参麦方/超高效液相色谱-飞行时间质谱/代谢组学/阿霉素/心肌损伤/作用机制Key words
Shen-Mai decoction/UPLC/TOF-MS/metabolomics/doxorubicin/myocardial injury/mecha-nism分类
医药卫生引用本文复制引用
李杰,高月,胡超,许龙龙,马增春,梁乾德,汤响林,王宇光,谭洪玲,肖成荣..参麦方对于大鼠心肌损伤保护作用的代谢组学研究[J].中国药理学通报,2016,32(11):1559-1564,1565,7.基金项目
国家自然科学基金资助项目( No 81274127) （ No 81274127）
国家重点基础研究发展计划资助项目( No 2012CB518402,2011CB505304) （ No 2012CB518402,2011CB505304）
