中华临床免疫和变态反应杂志2016，Vol.10Issue(3)：207-212,6.DOI:10.3969/j.issn.1673-8705.2016.03.005
人脐带间充质干细胞对难治性免疫性血小板减少症患者B淋巴细胞分化及分泌功能的影响
Impacts of hUC-MSCs on the Secretion and Immune Function of B Lymphocytes in Refractory Immune Thrombocytopenia Patients
摘要
Abstract
Objective To explore the impacts of hUC-MSCs on the secretion and immune function of B lymphocytes in refractory idiopathic thrombocytopenic purpura patients.Methods Enzyme digestion was used to extract healthy fetus umbilical cord mesenchymal stem cells; The 26 rITP patients who were in the Zhejiang Provincial Hospital of TCM from June 2012 to October 2013 and six healthy donors were included into this study, The peripheral blood mononuclear cells ( PBMCs) of them were extracted by density gradient centrifugation; Pokeweed ( PWM) was used to stimulate B lymphocyte transferring to plasmocytes for secretion of IgG and IgM.The concentration of IgG and IgM of the rITP patients were tested by enzyme-linked immuno sorbent assay ( ELISA) when their which PBMCs were co-cultured with hUC-MSCs, so did for healthy donors. The epression of Blimp-1 was measured by the EnVision immunohistochemistry.Results ( 1 ) The cells extracted from healthy fetus umbilical cord tested by flow cytometry labeled for CD44 was 99.8%. ( 2 ) The antibody IgG and IgM secreted from the rITP patients was ( 7.39 ±1.42 ) μg/L and ( 5.44 ±0.17 ) μg/L respectively, that was significantly increased than healthy doners, when the concentration of PWM was 30 mg/L and the cells were stimulated for 4 days (P=0.014, 0.039) .(3) When the hUC-MSCs and the PBMCs was at a 2∶10 ratio, the IgG and IgM concentration of rITP patients was (4.98 ±1.63) μg/L and (3.78 ±0.82) μg/L respectively, which was declined sharply than non-hUC-MSCs'( P =0.005, 0.003 ) , and not much difference with healthy donors ( P=0.848) .The rate of Blimp-1 protein of rITP patients was 33.51%, that apparently declined at the ratio 2∶10 of hUC-MSCs/peripheral blood mononuclear cells ( P =0.026), but no significant difference when compared to healthy donors (P=0.076).Conclusions The hUC-MSCs co-cultured with rITP patiens'peripheral blood mononuclear cells at 2∶10 ratio could decline IgG and IgM secretion and inhibit the expression of Blimp-1 protein.We suggest that hUC-MSCs affect on B lymphocytes of rITP patients and provides basis for the clinical application in rITP.However, this needs to be further studied before it is applied in clinical practice.关键词
血小板减少/间质干细胞/B淋巴细胞Key words
thrombocytopenia/mesenchymal stem cells/B-lymphocytes分类
医药卫生引用本文复制引用
曾玉晓,徐玲珑,吴迪炯,叶宝东,高雁婷,刘文宾,周郁鸿..人脐带间充质干细胞对难治性免疫性血小板减少症患者B淋巴细胞分化及分泌功能的影响[J].中华临床免疫和变态反应杂志,2016,10(3):207-212,6.基金项目
浙江省医药卫生科学研究项目（2010KYB077），国家自然科学基金（81373634），浙江省中医药优秀青年人才基金计划 （）
