中华临床免疫和变态反应杂志2016，Vol.10Issue(3)：242-246,5.DOI:10.3969/j.issn.1673-8705.2016.03.011
男性免疫性不育患者外周血T细胞亚型分布
Distribution of Peripheral Blood T Cells in Male Immune-Related Infertility
摘要
Abstract
Objective To analyze the distribution of T cells and Th cells in the peripheral blood of male immune-related infertility patients and to reveal the immune state of male immune-related infertility. Methods Flow cytometry was used to detect the distribution of T cell subsets in the peripheral blood of male immune-related infertility patients.Real-time fluorescence quantitative PCR ( RT-PCR) was used to detect the expression of IFN-γand IL-4, which were effector of Th1 and Th2 at the gene transcription level.Results The results of flow cytometry showed that the percentage of CD3 +T cells and CD8 +T cells in lymphocytes in immune-related infertility patients was significantly higher than that of normal controls.The percentage of CD4 +T cells in lymphocytes was significantly decreased, and the ratio of CD4/CD8 cells in immune-related infertility was decreased and inversion.The difference was statistically significant ( P<0.05 ) .RT-PCR test results showed that the expression of IFN-γmRNA in immune-related infertility group was significantly up-regulated, and the expression of IL-4 mRNA was significantly lower than that of normal control.The difference was statistically significant ( P <0.05 ) .Conclusion The immune system of male immune-related infertility has prominent T lymphocyte dysfunction and Th cell differentiation is shifted to Th1 direction and Th1 cell polarization.关键词
不育, 男 (雄) 性/T淋巴细胞亚群/Th细胞极化Key words
infertility, male/T-lymphocyte subsets/Th1 cell polarization分类
医药卫生引用本文复制引用
张晓霞,魏任雄,周俊,娄江涛..男性免疫性不育患者外周血T细胞亚型分布[J].中华临床免疫和变态反应杂志,2016,10(3):242-246,5.基金项目
宁波市自然基金（2013A610273），浙江省自然基金 （）
