安徽农业科学2016，Vol.44Issue(30)：70-71,92,3.
超高压高效液相色谱法测定茶酒中茶氨酸含量
Determination of Theanine in Tea Liquor by Ultra High Pressure and High Performance Liquid Chromatography
摘要
Abstract
Objective] The analytical method for content of theanine in tea liquor was investigated.[Method] Using o-phthalaldehyde (OPA) as a derivating agent,a method was established for determination of theanine in tea liquor by super high-pressure high performance liquid chromatog-raphy.[Result] The results showed that the linear range of 0.1-100.0 μg/mL for theanine detection was obtained,and the corresponding linear equation was fitted as Y=53.57X-41.664 with correlation coefficient r of 0.999 59.Also,the minimum detection limit of 0.05 μg/g (S/N=3) was reached.The theanine content of the sample was determined as 0.25 mg/kg with a recovery rate of 99.6%-100.1%.[Conclusion] The method is rapid,simple and accurate,which is easy to be widely used in the determination and analysis of theanine in tea liquor .关键词
超高压/高效液相色谱/茶酒/茶氨酸Key words
Ultra high pressure/High performance liquid chromatography/Tea liquor/Theanine分类
轻工纺织引用本文复制引用
杨威,吕超志,刘峰,李丹,曹忠..超高压高效液相色谱法测定茶酒中茶氨酸含量[J].安徽农业科学,2016,44(30):70-71,92,3.基金项目
国家自然科学基金项目（31527803）。 （）
