安徽农业科学2016，Vol.44Issue(30)：104-107,194,5.
LAT52启动子在甘蓝花药中的表达模式
Expression Profile of LAT52 Promoter in Anther of Brassica oleracea L.
摘要
Abstract
Objective] To verify whether specific promoter LAT52 could play a function in anther of cabbage.[Method] LAT52 core se-quence 608 bp was obtained from tomato by amplification technology, the sequence was analyzed, the LAT52 promoter was connected to the bi-nary expression vector pBI121, Agrobacterium GV3101 was transformed, Agrobacterium infection method was used to infect hypocotyl of cab-bage seedling, and 2 positive plants were obtained, transgenic cabbage plants flower bud, anther and pollen was stained by GUS.[Result] The LAT52 core sequence 608 bp had multiple pollen specific expression elements .The infected LAT52 promoter only expressed blue in the anther and pollen, and the other parts were not stained blue .[Conclusion] The LAT52 promoter can be expressed in anther and pollen of cabbage .关键词
LAT52启动子/甘蓝/花药/GUS/表达模式Key words
LAT52 promotor/Brassica oleracea L./Anther/GUS/Expression pattern分类
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于晓俊,曹绍玉,李翔,张颖,宋婷,董玉梅,毕保良,张应华,许俊强..LAT52启动子在甘蓝花药中的表达模式[J].安徽农业科学,2016,44(30):104-107,194,5.基金项目
国家自然科学基金项目（31560560）；云南省应用基础研究计划项目（2015FD019）。 （）
