安徽农业科学2016,Vol.44Issue(30):110-111,199,3.
何鲁牵牛的组培快繁研究
Study on Tissue Culture and Rapid Propagation of Ipomoea holubii
摘要
Abstract
Objective] To establish tissue rapid propagation system of Ipomoea holubii,and to provide theoretical foundation for its large-scale production.[Method] Taking the stem of Ipomoea holubii as explants,we researched the optimal culture medium for adventitious buds prolifera-tion and rooting of I.holubii.[Result] Axillary buds were induced on WPM+0.3 mg/L 6-BA +0.01 mg/L NAA +0.1 g/L active carbon (AC).The shoots of I.holubii were cultured on WPM +0.3 mg/L 6-BA +1 g/L AC for multiplication,and the multiplication ratio was 4.30. WPM +0.1 mg/L IBA+0.1 mg/L NAA+0.1 g/L AC was the optimal medium for rooting,and the rooting rate was 90%.[Conclusion] This re-search establishes the tissue culture and rapid propagation system of I.holubii,which is helpful to the germplasm protection and industrial produc-tion of I.holubii.关键词
何鲁牵牛/组培快繁/增殖/生根Key words
Ipomoea holubii/Rapid propagation/Multiplication/Rooting分类
农业科技引用本文复制引用
吕永平,王燕,牟豪杰,汪一婷,李海营..何鲁牵牛的组培快繁研究[J].安徽农业科学,2016,44(30):110-111,199,3.基金项目
国际先进农业科技术计划(948)项目(2011-G31);浙江省农业科学院青年人才培养项目(2015R21R08E08)。 ()