北京中医药大学学报2016，Vol.39Issue(10)：860-863,4.DOI:10.3969/j.issn.1006-2157.2016.10.015
葛根黄芩黄连方不同提取制备工艺的比较
Comparison of different extraction methods on Gegen Qinlian Decoction
摘要
Abstract
Objective By comparing the efficacy and toxicity of three extraction methods of Gegen Qinlian formula,so as to underline the appropriate technology of its extraction and preparation.Methods Mice were divided into blank group,pathological group,tablet group,pill group and decoction group, in which the latter three groups were divided into high -,mid -and low -dose subgroups respectively. The mice models were induced by i.g.senna decoction 20 mL/kg(4 g/kg).The tablet group were i.g. extract water solution at dose of 1.60,0.80,0.40 g/kg,the pill group were i.g.at dose of 6,3 and 1.5 g/kg,and the decoction group at dose of 13,6.5 and 3.25 g/kg.The number of the wet dung was chosen to evaluate the antidiarrheic effect of Gegen Qinlian formula.Then the acute toxicity test of differ-ent samples was carried out through maximal dosage experiments.Mice were divided into tablet group, pill group and decoction group,with 10 mice in each group.And the maximum dosage were 0.66 g/mL (tablet),0.63 g/mL(pill)and 0.74 g/mL(decoction),equivalent to crude drug pieces of 67.96, 89.01 and 177.77 g/kg,and equivalent to 24,9 and 6 times daily dose of per kilogram of body weight of 70 kg.After the single dose administration,the reactions of the mice were observed for consecutive seven days.Results The sample of three dosage forms of Gegen Qinlian formula had obvious therapeutic effect (P <0.001),among which the antidiarrheic effect of tablets was best (P <0.05),pill less and decoc-tion least.No death or obvious abnormalities was discovered under maximum dosage.Conclusion In the three preparations,Gegen Qinlian tablets extraction preparation method recorded in 2015 Chinese Pharmacopoeia had the best inhibitory effect against diarrhea caused by senna leaves.关键词
葛根芩连汤/药效/急性毒性/小鼠Key words
Gegen Qinlian prescription/therapeutic effect/acute toxicity/mice分类
医药卫生引用本文复制引用
罗静,白戌,古丽扎尔·阿布拉,李士远,金粟,王秀丽..葛根黄芩黄连方不同提取制备工艺的比较[J].北京中医药大学学报,2016,39(10):860-863,4.基金项目
2015年北京市共建项目 （）
