草业学报2016，Vol.25Issue(11)：34-42,9.DOI:10.11686/cyxb2016011
豌豆白粉菌侵染对活性氧迸发规律和紫花苜蓿叶片结构的影响
The effect of Erysiphe pisi infection on the pattern of oxidative burst and on anatomic structure of leaves in Medicago sativa
摘要
Abstract
Medicago sativa was inoculated with Erysiphe pisi .The colorimetric method was used to study the timing and intensity of · O2 - and H2 O2 bursts in leaves,and histochemical methods were used to study locali-zation of · O2 - and H2 O2 accumulation.In addition,paraffin sections were made from infected leaves to ob-serve structural changes in those leaves.It was found that after inoculation,· O2 - and H2 O2 bursts in Qingy-ang alfalfa (resistant cultivar)were observed as two peaks,appearing 4 and 48 h after inoculation for · O2 - and 4 and 24 h after inoculation for H2 O2 ,respectively,with the intensity of the first peak higher than that of the second peak.By contrast,in Debao alfalfa (susceptible cultivar),no obvious fluctuation in · O2 - content ap-peared and only the one peak for H2 O2 accumulation was seen.Staining to reveal zones of localization of reac-tive oxygen species (ROS)showed that blue-stained · O2 - deposition sites did not occur at the surface of or in-side of leaves,while brown-stained H2 O2 deposition sites were observed in the cell wall of upper and lower epi-dermal cells,and the cytoplasm of infected mesophyll cells.Most importantly,we report here for the first time that the tissue structure of alfalfa leaves was changed after infection with E .pisi.Palisade cells changed from a long-cylindrical cell type to groups of several subrotund cells,so that it was difficult to distinguish the palisade tissue and spongy tissue cells.Our results show that for the different plant species and pathogen resistance combinations investigated,the timing and intensity of ROS accumulation differed,as did the identity of the chemical species generated in activating the defense reaction.The structural and morphological change of meso-phyll cells may be one component of an effective response against powdery mildew infection in M.sativa.关键词
过氧化氢/超氧阴离子/过敏性反应/解剖结构Key words
hydrogen peroxide/superoxide anion/hypersensitive response/anatomic structure引用本文复制引用
张咏梅,马晖玲,唐云智..豌豆白粉菌侵染对活性氧迸发规律和紫花苜蓿叶片结构的影响[J].草业学报,2016,25(11):34-42,9.基金项目
甘肃农业大学盛彤笙科技创新基金（GSAU-STS-1343）和“十二五”农村领域国家科技计划专题（2012BAD12B02-4）资助。 （）
