草业学报2016，Vol.25Issue(11)：43-49,7.DOI:10.11686/cyxb2016158
日本结缕草 ZjADH 基因对拟南芥的转化及其耐寒性分析
Transformation of ZjADH gene into Arabidopsis thaliana and cold-tolerance analysis of transgenic plants
摘要
Abstract
To study the relationship between the ZjADH gene and plant cold tolerance,a plant expression vec-tor 3302Y-ZjADH was constructed through DNA recombination technology.Transgenic Arabidopsis thaliana plants with glufosiante resistance were generated by floral dip method.PCR and qRT-PCR results showed that the target gene was successfully integrated with the genomes of Arabidopsis and could express efficiently. Both wild type and transgenic Arabidopsis plants were subjected to 4 ℃ to investigate their cold tolerance. Tests demonstrated that,compared with the wild type,the transgenic plants were more tolerant of cold stress. The free proline content in transgenic plants was significantly higher than the control,while the accumulation of ROS was much lower than the wild type.In addition,the expression levels of genes related to stresses like SOD ,APX and LEA in transgenic plants were much higher than in the control.Results indicated that over-ex-pression of ZjADH may improve the activities of superoxide dismutase (SOD),ascorbate peroxidase (APX) and late embryogenesis abundant proteins (LEA)to improve the cold tolerance of transgenic plants.In conclu-sion,the heterologous expression of ZjADH genes could reinforce the resistance of plants to cold stress.This study provides a theoretical foundation for future research on the ZjADH gene and the cultivation of cold-toler-ant Zoysia japonica cultivars.关键词
ZjADH 基因/转基因拟南芥/低温胁迫/脯氨酸/活性氧Key words
ZjADH gene/transgenic Arabidopsis thaliana/cold stress/proline/ROS引用本文复制引用
张兰,滕珂,肖国增,梁小红,许立新,尹淑霞,晁跃辉..日本结缕草 ZjADH 基因对拟南芥的转化及其耐寒性分析[J].草业学报,2016,25(11):43-49,7.基金项目
北京林业大学科技创新计划项目（BLX2014-06），国家高技术研究发展计划（863计划）（2013AA102607）和中国林学会－青年人才托举工程资助。 （）
