草业学报2016，Vol.25Issue(11)：57-65,9.DOI:10.11686/cyxb2015591
NO 介导的 Ca2＋信号在干旱胁迫下紫花苜蓿种子萌发及抗氧化酶中的传导作用研究
Investigation into the mechanism of Mo-mediated Ca2+ signaling during seed germination and antioxidation in Medicago sativa under drought stress
摘要
Abstract
The Ca2 + transduction pathways have been implicated in mediating stress response and tolerance in plants.In order to investigate the mechanism of Ca2 + signaling mediated by nitric oxide (NO)during seed ger-mination and antioxidation in Medicago sativa under drought stress,sodium nitroprusside (SNP,nitric oxide donor),CaCl2 ,and methylene blue (NO blockers)and LaCl3 (Ca2 + channel blockers)were used in this study, and alfalfa seeds were pre-soaked with these solutions.An index of germination was markedly decreased under drought stress induced by 15% polyethylene glycol treatment,but this effect was reversed after treatments with SNP and Ca2 + .Moreover,0.1 mmol/L SNP or 10 mmol/L CaCl2 alleviated drought stress damage.Com-pared to SNP or CaCl2 treatment alone,germination rate significantly increased by 8.96% and 19.67% respec-tively when the seeds were treated with both SNP and Ca2 + .Furthermore,both SNP and CaCl2 treatments in-creased content of starch,soluble sugar,soluble protein and proline,and activities of amylase,superoxide dis-mutase,peroxidase and catalase,whereas malondialdehyde content decreased compared to that under SNP or CaCl2 treatment alone.The changes were slower for seeds receiving both NO and Ca2 + treatments than for NO or Ca2 + treatment alone.Interestingly,with added exogenous Ca2 + and methylene blue,the promotional effect of Ca2 + was inhibited.In addition,the promotional effect of NO was inhibited by La3 + .This indicates that NO mediated protein modifications in alfalfa seeds under drought stress through the Ca2 + signaling pathway.关键词
一氧化氮/钙离子/紫花苜蓿/干旱胁迫/种子萌发/抗氧化酶Key words
nitric oxide/Ca2 +/alfalfa/drought stress/seed germination/antioxidant enzymes引用本文复制引用
弋钦,魏小红,强旭,赵颖,丁春发..NO 介导的 Ca2＋信号在干旱胁迫下紫花苜蓿种子萌发及抗氧化酶中的传导作用研究[J].草业学报,2016,25(11):57-65,9.基金项目
国家自然基金项目（31560663）和甘肃省自然基金项目（145RJZA196）资助。 （）
