福建农业学报2016，Vol.31Issue(9)：912-916,5.DOI:10.19303/j.issn.1008-0384.2016.09.003
创伤弧菌铁调基因fur的原核表达及多克隆抗体制备
Prokaryotic Expression and Polyclonal Antibody for Ferric Uptake Regulator Prepared from Vibrio vulnificus FJ0 3-X2
摘要
Abstract
Ferric uptake regulator (fur)gene from Vibrio vulnificus FJ03-X2 was cloned,and inserted into the prokaryotic expression vector,pET32a. Coding sequence of the gene contained 450 bps,encoding 149 amino acids. Its sequencing showed a greater than 98% homology with that of the fur gene from GenBank. Subsequently,the plasmid,pET32a-FUR,was transformed into E.coli BL21 to express the recombinant protein,rFUR. The soluble rFUR was then subject to the Ni-NTA His Binding affinity purification. The purified rFUR was injected into SD rats to produce polyclonal antibody. The obtained highly specific antibodies of FUR was confirmed by ELISA assay.关键词
创伤弧菌/铁调基因fur/克隆/原核表达/多克隆抗体Key words
Vibrio vulnificus/ferric uptake regulator/cloning/prokaryotic expression/polyclonal antibody分类
农业科技引用本文复制引用
李素一,张丽娟,陈华,柯翎,陈叙,林晨韬..创伤弧菌铁调基因fur的原核表达及多克隆抗体制备[J].福建农业学报,2016,31(9):912-916,5.基金项目
福建省科技计划项目---省属公益类科研院所基本科研专项（2014R1019-9) （）
福建省农业科学院引进海外人才科研启动基金项目（HWRC2011-03、HWRC2011-02) （）
国家自然科学基金项目（31100658) （）
福建省农业科学院青年英才计划项目（YC2015-20) （）
福建省农业科学院杰出青年人才基金项目（2014JQ-4) （）
