福建农业学报2016，Vol.31Issue(9)：933-938,6.DOI:10.19303/j.issn.1008-0384.2016.09.007
青花菜BoBURP2基因的克隆与表达分析
Cloning and Expression Analysis on BoBURP2 Gene of Brassica oleracea var. italica
摘要
Abstract
A plant-specific protein,BURP plays an important role in the growth,development,and stress response of a plant. In this study,a BURP gene designated as BoBURP2 was isolated from broccoli,Brassica oleracea var. italica,using PCR. The gene sequencing showed its genome DNA was 1 2 1 8 bp with a single intron. The complete coding sequence was 840 bp in length,encoding 279 amino acids. It shared a high homology with RD22,which is also a member of the BURP family. Phylogenetic analysis on BoBURP2 showed its sequence to be highly similar to thoseofB.oleracea,B.rapa,andB.napus.Theycouldpossiblybegroupedinasameclade.Ontheotherhand, the low similarity it shared with the sequence of Tarenaya hassleriana suggested a remote relationship between them. The RT-PCR results indicated the expression of BoBRUP2 was induced by both NaCl and drought stresses, with the highest levels detected at 24 h and 48 h. The cloning and expression analysis on BoBURP2 provided the information for further studies on the identification of its genetic function as well as the molecular breeding for stress resistant B. oleracea var. italica.关键词
青花菜/BoBURP2/RD22/克隆/表达Key words
Brassicaoleracea var.italica/BoBURP2/RD22/cloning/expression分类
生物科学引用本文复制引用
章燕如,许鑫,祝琦,周秀倩,俞可可,龚秀,蒋明..青花菜BoBURP2基因的克隆与表达分析[J].福建农业学报,2016,31(9):933-938,6.基金项目
国家级大学生创新创业训练计划项目（201510350011) （）
浙江省公益性技术应用研究计划项目（2016C32091) （）
台州市科技计划项目（162ny14) （）
