福建农业学报2016，Vol.31Issue(9)：986-992,7.DOI:10.19303/j.issn.1008-0384.2016.09.017
应用PCR-DGGE技术分析福州左海湖的细菌群落组成
Composition of Bacterial Community at Lake Zuohai Determined by PCR-Denaturing Gradient Gel Electrophoresis
摘要
Abstract
Composition and predominant groups of the bacterial community in Lake Zouhai in the city of Fuzhou were determined and analyzed by using PCR-DGGE. Water samples were collected from 4 sites each time in December 2011,February 2012,April 2012,and June 2012. The results showed that the water samples from the different sites on a same date had a similar bacterial composition,while the numbers and positions of DGGE profiles indicated significant compositional differences on different dates. Twenty DGGE bands with varied phylotypes were excised, cloned,sequenced,and analyzed to show 11 of them having possible affiliations with Proteobacteria,5 with Bacteroidetes,two with Actinobacteria, and two with Cyanobacteria. Analysis on the bacterial compositions suggested that Proteobacteria was the predominant group existed in the lake.关键词
左海湖/细菌群落组成/16SrRNA/PCR-DGGEKey words
lake Zuohai/bacterial community composition/16S rRNA/PCR-DGGE of sampling sites分类
生物科学引用本文复制引用
吕新,刘兰英,陈丽华,李玥仁,林碧娇..应用PCR-DGGE技术分析福州左海湖的细菌群落组成[J].福建农业学报,2016,31(9):986-992,7.基金项目
福建省自然科学基金项目（2011J01118) （）
福建省科技计划项目---省属公益类科研院所基本科研专项（2015R1025-1) （）
福建省财政专项---福建省农业科学院科技创新团队建设项目（2016P1-18) （）
福建省农业科学院青年人才创新基金（2014QB-31) （）
