医学分子生物学杂志2016，Vol.13Issue(6)：329-335,7.DOI:10.3870/j.issn.1672-8009.2016.00.000
糖脂毒性作用下小鼠胰岛miRNA的表达谱变化
Effect of Glycolipid Toxicity on miRNA Expression Profile in Murine Pancreatic Islets
摘要
Abstract
Objective To explore the effect of long-term high-glucose and/or high-fat culture on the miRNAs expression in mouse primary pancreatic islets. Methods RNA was extracted from the primary pancreatic islets obtained from B6 mice. MiRNAs that experienced great changes after high-glucose and/or high-fat culture were detected by high-throughput biochip microarray and RT-PCR. The bioinformatics method was used to predict the downstream target genes of miRNAs and the results were verified by western blotting. Results A variety of miRNAs changed significantly in the primary islets after high-glucose and/or high-fat culture, and miR-320 was found to increase under the culture of high glucose and high fat. p85α was predicted to be a downstream target gene of miR320 by bioinformatics method. Western blotting showed that p85α was significantly decreased in pancreatic isletβcells after miR-320 was inhibited. Conclusion The expression of miR-320 is sig-nificantly increased in primary pancreatic islets under high-glucose and/or high-fat culture, sugges-ting that miR-320 may be involved in the development of type 2 diabetes by regulating the immune system.关键词
糖脂毒性/原代胰岛/miRNAKey words
glycolipid toxicity/primary islets/miRNA分类
医药卫生引用本文复制引用
吴倜珺,许茹凤,韩晓,严虹..糖脂毒性作用下小鼠胰岛miRNA的表达谱变化[J].医学分子生物学杂志,2016,13(6):329-335,7.基金项目
南京市卫生局医学科技发展基金 （）
