国际生殖健康计划生育杂志2016，Vol.35Issue(6)：454-458,5.
α亚麻酸对体外成熟人卵母细胞线粒体DNA拷贝数的影响
Effect of Linolenic Acid on Mitochondrial DNA Copy Numbers of Human Oocyte Derived from in Vitro Maturation
摘要
Abstract
Objective: To explore the effect of alpha-linolenic acid (ALA) on human oocytes maturation and the mitochondrial DNA (mtDNA) copy numbers of oocyte. Methods: A total of 441 morphologically normal immature oocytes were recruited from 273 infertile women underwent ICSI in our Center from October 2014 to October 2015. There were 179 oocytes at MⅠ stage and 262 oocytes at GⅤ stage. Oocytes with the same proportion of MⅠand GⅤwere randomly divided into 5 groups, and in vitro cultured for 24 hours in the medium with ALA at different level [0 (as control), 10 μmol/L, 50 μmol/L, 100 μmol/L, 200 μmol/L]. The oocyte maturation was observed, and the mtDNA copy numbers in the ALA 0μmol/L (as control) group and the ALA 50μmol/L group were determined by real-time PCR. Results: ①The total maturation rate of the ALA 50 μmol/L group (70.1%) was significantly higher than that of the control group (42.1%) and the ALA 200 μmol/L group (34.1%, both P<0.05). ②The total maturation rate of GⅤ in the ALA 50 μmol/L group (64.4%) was significantly higher than that in the control group (28.9%) and the ALA 200 μmol/L group (18.2%, both P<0.05).③The mtDNA copy number of in vitro matured oocytes in the ALA 50μmol/L group (3.64×107±1.85×106) was significantly higher than that in the ALA 0 μmol/L group (3.42×106±1.95×105, P<0.05). Conclusions: 50μmol/L ALA can increase the mtDNA copy number of the in vitro matured human oocyte, suggesting that ALA can promote the in vitro maturation of human oocyte.关键词
α亚麻酸/卵母细胞/细胞培养技术/DNA,线粒体Key words
Alpha-linolenic acid/Oocytes/Cell culture techniques/DNA, mitochondrial引用本文复制引用
胡继君,孙丽君,管一春,郝大勇,马晓娟,齐若凡,董孝贞..α亚麻酸对体外成熟人卵母细胞线粒体DNA拷贝数的影响[J].国际生殖健康计划生育杂志,2016,35(6):454-458,5.基金项目
2014年度河南省医学科技攻关计划普通项目 （）
