郑州大学学报（医学版）2016，Vol.51Issue(6)：690-694,695,6.DOI:10.13705/j.issn.1671-6825.2016.06.002
下调 Ets2表达对裸鼠食管鳞癌移植瘤生长及 mTOR／p70S6K信号通路的影响
Effect of downregulating Ets 2 on tumor growth and mTOR/p70S6K signa-ling pathway in nude mice xenografted esophageal squamous cell carcinoma
摘要
Abstract
Aim:To observe the effect of downregulating expression of Ets 2 on the tumor growth and mTOR/p70S6K signaling pathway in nude mice xenografted Eca 109 tumor.Methods:The siRNA which could down-regulate the expression of Ets2 effectively in vitro had been selected before .LV containing the siRNA ( LV-siEts2 ) was established and infected Eca109 .Fifteen nude mice were randomly allocated into control group , negative control group and experimental group . Eca109, Eca109 infected by LV and Eca109 infected by LV-siEts2 were inoculated subcutaneously into nude mice to estab-lish transplantation tumor .Later,we observed tumor growth , drawn the tumor growth curve and calculated the tumor inhibi-tion rate.The expression of Ets2 mRNA in tumor tissue was detected by qRT-PCR and cell apoptosis was detected by TUNEL.The protein expression of Ets2,Caspase-3,Bcl-2,E-cadherin and mTOR/p70S6K signaling pathway in tumor tis-sue was detected by Western blot .Results:The growth rate of tumors in the experimental group slowed down significantly and tumor size and weight was less than the control group and negative control group (P<0.05),besides,the mRNA and protein expression of Ets2 in tumor tissue from the experimental group was reduced significantly .The results of TUNEL demonstrated that LV-siEts2 could significantly induce apoptosis of Eca 109 cells(P<0.05).The protein expression levels of Caspase-3 and E-cadherin increased and the protein expression levels of Bcl-2 decreased.In addition, The results of Western blot demonstrated that LV-siEts2 could down-regulate the protein expression level of mTOR and p 70S6K signifi-cantly.Conclusion:LV-siEts2 could promote cell apoptosis by inhibiting mTOR/p70S6K signaling pathway in vivo , thus inhibiting tumor growth in nude mice xenografted Eca 109 tumor.关键词
Ets2/siRNA/Eca109/裸鼠/凋亡/mTOR/p70S6K信号通路Key words
Ets2/siRNA/Eca109/nude mice/apoptosis/mTOR/p70S6K signaling pathway分类
医药卫生引用本文复制引用
韩康,马珊珊,李庆华,王欣欣,朱相展,孟楠,韩莉,关方霞..下调 Ets2表达对裸鼠食管鳞癌移植瘤生长及 mTOR／p70S6K信号通路的影响[J].郑州大学学报（医学版）,2016,51(6):690-694,695,6.基金项目
国家自然科学基金项目81471306，U1404313，河南省高等学校重点科研项目15A310028 （）
