郑州大学学报（医学版）2016，Vol.51Issue(6)：695-699,5.DOI:10.13705/j.issn.1671-6825.2016.06.003
β-胡萝卜素对食管癌细胞凋亡与 Cav-1表达的影响
Relationship between β-carotene inducing-apoptosis and Cav-1 expres-sion in human esophageal squamous cancer cells
摘要
Abstract
Aim:To observe the effect of β-carotene on cell apoptosis in esophageal cancer cells , and analyze the rela-tionship between the sensitivity of esophageal cancer cells to β-carotene and the expression of caveolin-1(Cav-1).Methods:The expressions of Cav-1 protein in normal esophageal epithelial cell line Het-1A and three esophageal cancer cell lines ( TE1, EC1,Eca109) were detected by Western blot; after treatment with different concentrations (0,1, 5, 10, 20, 30 and 50μmol/L) of β-carotene for 12, 24, 36, 48, 60 and 72 h, the proliferation rate of esophageal cancer cells was tested by CCK 8 assay, and the optimal time and concentration of β-carotene were determined; then the cell apoptosis of esophageal cancer cells were detected by Annexin V-FITC/PI staining;the mRNA level of Cav-1 was determined by qRT-PCR, the expression level of Cav-1 protein was detected by Western blot .Results:Cav-1 was over-expressed in esophageal cancer cells ( TE1>Eca109>EC1), and was undetectable in Het-1A.CCK-8 assay showed that β-carotene has no effect on Het -1A cells, but in-hibited the proliferation of three esophageal cancer cell lines in a dose-and time-dependent manner .In addition, there were relation between the expression of Cav-1 and the sensitivity of esophageal cancer cells to β-carotene.The dose and treatment time ofβ-carotene when the cell proliferations were decreased nearly 50%were used in the following experiments .After treat-ment with β-carotene, TE1,EC1 and Eca109 cells had higher apoptosis rate than that of negative control group (P<0.05), especially TE1.In addition, the mRNA and protein level of Cav-1 were both significantly downr-egulated.Conclusion :β-carotene can effectively induce apoptosis in esophageal cancer cells , which is nont-oxic to the normal esophageal epithelial cell.Moreover , the sensitivity of esophageal cancer cells to the β-carotene may be regulated by Cav1-.关键词
β-胡萝卜素/食管癌/小窝蛋白-1/细胞凋亡/TE1/EC1/Eca109Key words
β-carotene/esophageal cancer/caveolin-1/cell apoptosis/TE1/EC1/Eca109分类
医药卫生引用本文复制引用
朱相展,张彦婷,韩康,李庆华,张楠楠,马珊珊,张琨,关方霞..β-胡萝卜素对食管癌细胞凋亡与 Cav-1表达的影响[J].郑州大学学报（医学版）,2016,51(6):695-699,5.基金项目
国家自然科学基金项目81071008，81471306，U1404313；河南省科技创新人才计划154200510008；河南省高校科技创新团队15IRTSTHN022；河南省产学研合作项目142107000008 （）
