郑州大学学报（医学版）2016，Vol.51Issue(6)：700-703,4.DOI:10.13705/j.issn.1671-6825.2016.06.004
RNA 干扰人永生化食管上皮恶变细胞中核干细胞因子对PI3K／AKT表达的影响
RNA interfering effect of nucleostemin on expression of PI 3 K/AKT in Shantou human embryonic esophageal carcinoma epithelial cells
摘要
Abstract
Aim: To explore the effect of inhibiting nucleostemin (NS) on PI3K/AKT signal pathway in SHEEC. Methods:RNA interfering agents ( NS-RNAi) was transfected into SHEEC to inhibit the expression of NS gene , with the SHEEC group, negative control(NC) group, and MOCK group as control, and the expressions of PI3K/AKT pathway relat-ed factors were detected by using RT-PCR and Western blot methods .Results: SHEEC was transfected by NS-RNAi and the transfection rate was high.The expression levels of PI3K and AKT mRNA[(0.460 ±0.207) and (0.492 ±0.233)] were obviously down-regulated by inhibiting NS gene in comparison with SHEEC , NC and MOCK groups(P<0.05).The expression levels of PI3K and AKT proteins were (0.353 ±0.361) and (0.312 ±0.068), and showed significant differ-ence in comparison with SHEEC, NC and MOCK groups(P<0.05).Conclusion: The expressions of PI3K/AKT signal pathway related factors decrease with NS down-regulation in SHEEC , and the PI3K/AKT signal pathway may be a kind of NS P53-independent pathway .关键词
永生化食管上皮恶变细胞/核干细胞因子/RNA干扰/PI3K/AKT信号转导通路Key words
SHEEC/nucleostemin/RNA interfering/PI3K/AKT signaling pathway分类
医药卫生引用本文复制引用
胡梦竹,王娟,王盼盼,张洪磊,张霞,张巧,高社干,张功员..RNA 干扰人永生化食管上皮恶变细胞中核干细胞因子对PI3K／AKT表达的影响[J].郑州大学学报（医学版）,2016,51(6):700-703,4.基金项目
河南省基础研究资助项目132300410129；河南省教育厅科技攻关资助项目13A330632 （）
