郑州大学学报（医学版）2016，Vol.51Issue(6)：723-726,4.DOI:10.13705/j.issn.1671-6825.2016.06.009
siRNA沉默 ARK5基因对 SMMC-7721细胞侵袭及 MMP-2蛋白分泌的影响
Effect of siRNA silencing ARK5 gene on invasion and secretion of MMP-2 of SMMC-7721 cells
蒋清虎 1马吉安 1谭孝华 1吴忠均2
作者信息
- 1. 达州市中西医结合医院肝胆外科达州635000
- 2. 重庆医科大学附属第一医院肝胆外科重庆400016
- 折叠
摘要
Abstract
Aim:To study the effect of ARK5 gene silencing on the invasion of SMMC-7721 cells.Methods:The ex-pression of ARK5 was determined by qRT-PCR and Western blot in SMMC-7721 and LO2 cells,respectively.ARK5 siRNA was constructed and transfected into SMMC-7721 cells, and negative group and blank group were set up;the expression of ARK5 protein was determined by Western blot , and the invasion of cells in 3 groups were examined by Transwell assay . MMP-2 protein in culture medium was determined by ELISA .Results:The mRNA and protein expression levels of ARK5 in SMMC-7721 cells were significantly higher than those in LO2 cells(P<0.05).After ARK5 siRNA transfection, the in-vasion of SMMC-7721 cells was significantly inhibited ,and MMP-2 protein secreted by the cells was significantly lower than those in negative group and blank group (P<0.05).Conclusion: ARK5 silencing could effectively inhibit invasion of SMMC-7721 cells via MMP-2 pathway.关键词
肝癌/侵袭/ARK5基因/RNA干扰/SMMC-7721细胞Key words
hepatocellular carcinoma/invasion/ARK5/RNA interference/SMMC-7721 cell分类
医药卫生引用本文复制引用
蒋清虎,马吉安,谭孝华,吴忠均..siRNA沉默 ARK5基因对 SMMC-7721细胞侵袭及 MMP-2蛋白分泌的影响[J].郑州大学学报（医学版）,2016,51(6):723-726,4.
