集成技术2016，Vol.5Issue(6)：79-84,6.
7种牛奶蛋白基因在大肠杆菌中的异源表达
Synthesis of Seven Milk Proteins inEscherichia Coli
摘要
Abstract
Seven main cow milk protein genes, includingαs1CN,αs2CN,βCN,κCN,αLa,βLg, and BSA (Bovine Serum Albumin), were expressed inEscherichia coli and three whey proteins were examined by western-blot. As a trial substage, these genes of seven cow milk protiens expressed successfully inE. coli wihout a phenomenon of degradation. The results indicated that it is initial feasible to gain recombinant milk proteins using recombinantE. coli, and this work lays foundations for the production of cow free milk.关键词
大肠杆菌/western-blot/重组牛奶蛋白Key words
Escherichia coli/westein-blot/recombinant milk proteins分类
矿业与冶金引用本文复制引用
张齐,崔金明,蒙海林,张炳照,魏婷,黄建东,刘陈立..7种牛奶蛋白基因在大肠杆菌中的异源表达[J].集成技术,2016,5(6):79-84,6.基金项目
广州市科技计划项目(201508020092)；深圳市未来产业发展专项资金(海洋类)第三批扶持计划(CXZZ20140901004122088)；广东省科技计划项目(2014A020216029、2014B020201001、2014A030304008、2014B050505003)；深圳科创委基础研究(JCYJ20150521144320992)；深圳市科技创新委员会孔雀团队(KQTD2015033117210153) （201508020092）
