检验医学与临床2016，Vol.13Issue(22)：3133-3134,3137,3.DOI:10.3969/j.issn.1672-9455.2016.22.001
RQ-PCR 在慢性粒细胞白血病细胞 BCR-ABL融合基因检测中的应用
Application of real-time quantification polymerase chain reaction in detecting the expression of BCR-ABL in chronic myelogenous leukemia cells
摘要
Abstract
Objective To explore the clinical significance of real‐time quantitative polymerase chain reaction (RQ‐PCR) in detec‐ting BCR‐ABLp210 fusion gene in chronic myelogenous leukemia (CML ) cells .Methods The transcript level of BCR‐ABLp210 was measured by RQ‐PCR from the bone marrow cells of 60 CML patients .Results All of 60 CML patients were BCR‐ABL posi‐tive .Among the 60 patients ,33 patients were in chronic phase ,13 patients were in accelerated phase and 14 patients were in blast phase .In contrast ,the transcript level of BCR‐ABLp210 in blast phase was significantly higher than that in the other phases .Six months after the treatment with allogenic hematopoietic stem cell transplantation (allo‐HSCT ) or imatinib ,the transcript level of BCR‐ABLp210 was decreased .In addition ,there was no significant difference between allo‐HSCT and imatinib treatment after 12 months(P > 0 .05) .Meanwhile ,the application of the other tyrosine kinase inhibitors had the similar results for CML after 12‐month treatment .Conclusion The detection of BCR‐ABLp210 transcript level by RQ‐PCR contributes to the diagnosis ,the evalua‐tion of curative effect and the minimal residue detection .关键词
慢性粒细胞白血病/BCR-ABL/实时荧光定量逆转录聚合酶链反应Key words
chronic myelogenous leukemia/BCR-ABL/real-time quantification polymerase chain reaction引用本文复制引用
吴薇,韩瑞玲,张力,李艳..RQ-PCR 在慢性粒细胞白血病细胞 BCR-ABL融合基因检测中的应用[J].检验医学与临床,2016,13(22):3133-3134,3137,3.基金项目
国家自然科学基金资助项目（81200389，81501427）；教育部高等学校博士学科点专项科研基金（20120141120077）；湖北省自然科学基金资助项目（2016CFB456）。 （）
