检验医学与临床2016，Vol.13Issue(22)：3135-3137,3.DOI:10.3969/j.issn.1672-9455.2016.22.002
中毒家兔体内呋喃丹水平检测及其体内分布规律研究
Study on the detection method and distribution of carbofuran in poisoned rabbits
摘要
Abstract
Objective To establish the method of the hydrophilic solid phase extraction material for extracting carbofuran .And to study the distribution of carbofuran in postmortem rabbits .Methods Twelve male rabbits were randomly allocated to 2LD50 and 4LD50 groups ,which were given an intragastric administration of carbofuran with a dose of 220 mg/kg and 440 mg /kg ,respectively . Vital signs and symptoms of poisoning were observed from given the medication to death .As soon as the vital signs disappeared ,the rabbits were dissected and the specimens such as heart blood ,heart ,liver ,spleen ,lung ,kidney ,brain .A hydrophilic solid phase ex‐traction column material was used to extract carbofuran ,and dichloromethane was used to elute carbofuran ,in addition ,gas chroma‐tographic was used to detect level of carbofuran .Results Recovery rate of hydrophilic solid phase extraction column to extract ma ‐terial was 99 .3% ,the detection limit was 5 ng/mL .The order of levels of carbofuran in organs was as follows :in 2LD50 group ,lung was (10 .98 ± 2 .30)μg/mL ,liver was (7 .92 ± 2 .39)μg/mL ,heart blood was (2 .13 ± 1 .36)μg /mL ,brain was (2 .01 ± 1 .57)μg/mL ,heart was (1 .23 ± 0 .38)μg/mL ,kidney was (1 .31 ± 0 .42)μg/mL and spleen was (0 .59 ± 0 .21)μg /mL .In 4LD50 group :lung was(6 .86 ± 3 .02)μg/mL ,liver was(6 .12 ± 2 .38)μg/mL ,kidney was (2 .52 ± 1 .54)μg/mL ,brain was(1 .91 ± 0 .72)μg/mL ,heart was(1 .48 ± 1 .75)μg/mL ,heart blood was(1 .17 ± 1 .57)μg/mL and spleen was(1 .15 ± 0 .66)μg/mL .Conclusion Hydrophilic solid phase for extracting carbofuran costs lower ,and it is more convenient ,which has higher extraction rate with lower impurity content , which can be applied in forensic medicine .关键词
亲水性固相材料/呋喃丹/分布/气相色谱Key words
hydrophilic solid phase material/carbofuran/distribution/gas chromatography引用本文复制引用
王勇,吕晓革,王小华,吴玉红,贾硕果,封宇,吴伟,卜娟..中毒家兔体内呋喃丹水平检测及其体内分布规律研究[J].检验医学与临床,2016,13(22):3135-3137,3.基金项目
重庆市高校优秀成果转化项目（KJZH14109）。 （）
