国际眼科杂志2016，Vol.16Issue(12)：2195-2199,5.DOI:10.3980/j.issn.1672-5123.2016.12.06
右归丸对糖尿病视网膜病变大鼠 Pl3 K/Akt 信号通路的影响
Effects of a traditional Chinese patent medicine for PI3 K/Akt pro-survival signal channel in diabetic retinopathy rat
摘要
Abstract
•AIM: To investigate the effects of reinforcing yang from yin of a traditional Chinese patent medicine called YouguiWan for PI3K/Akt pro-survival signal channel in diabetic retinopathy rat.•METHODS:A total of 80 SPF SD rats were selected, 10 rats were selected to set up normal group. The remaining 70 rats were given streptozotocin (60mg/kg) peritoneal injection combined with intravitreal injection of VEGF ( 0. 05μg ) to establish proliferative diabetic retinopathy rat model. After the model successful, the rats were divided into four groups: YouguiWan high dose group, YouguiWan middle dose group, YouguiWan low dose group and model group. The treatment groups were fed daily YouguiWan concentrate, model group and the normal group received daily doses of distilled water. <br> After intragastric administration for 3mo, the PI3K, Akt expression of retinal tissue were observed by SP -immunohistochemistry and Western - blot method respectively.•RESULTS: Immunohistochemical observation showed PI3K, Akt expression immunohistochemical staining, were brown particles on the retina. Normal PI3K, Akt expression weakly positive immunological reaction unit located in the ganglion cell layer. The kernel was also a small amount of expression. Model group, each YouguiWan treatment group rat retinal ganglion cell layer, inner plexiform layer and the inner and outer layer of the particles had a positive expression. Compared with the model group, YouguiWan high dose group PI3K, Akt expression were decreased. YouguiWan low dose group decreased expression was not obvious. Compared with the low dose group, YouguiWan high dose group PI3K, Akt expression was decreased. The expression of the strength of the YouguiWan, high dose group showed no significant difference. Western-blot test results showed that compared with the normal group, model group, treatment group, the expression difference was statistically significant (P<0. 01). Model group, treatment group, PI3K, Akt protein levels were significantly increased. Compared with the model group, the YouguiWan high - dose group was statistically significant (P<0. 05). YouguiWan high dose group PI3K, Akt protein expression were decreased, YouguiWan low dose group was no significant difference ( P> 0. 05 ). Compared with the low dose group, YouguiWan high-dose group was statistically significant ( P < 0. 05 ). YouguiWan high dose group PI3K, Akt expression levels were decreased. YouguiWan high dose group was not statistically significant difference (P>0. 05).• CONCLUSION: Reinforcing yang from yin of YouguiWan can affect PI3K, Akt protein expression and inhibit PI3K/Akt signaling pathway, delay the disease process of diabetic retinopathy, propose new ideas and scientific basis for the treatment of diabetic retinopathy and blindness prevention treatment.关键词
右归丸/阴中求阳法/糖尿病视网膜病变/PI3 K/AktKey words
YouguiWan/reinforcing yang from yin/diabetic retinopathy/PI3K/Akt引用本文复制引用
李欢,罗向霞,冯玉沛,王晗..右归丸对糖尿病视网膜病变大鼠 Pl3 K/Akt 信号通路的影响[J].国际眼科杂志,2016,16(12):2195-2199,5.基金项目
国家自然科学基金项目(No.81460685)@@@@National Nature Science Foundation Project (No.81460685) （No.81460685）
