山东农业科学2016，Vol.48Issue(11)：1-8,8.DOI:10.14083/j.issn.1001-4942.2016.11.001
三个小麦防御素基因的克隆及序列分析
Cloning and Sequence Analysis of Three Defensin Genes in Wheat (Triticum aestivum L.)
摘要
Abstract
Three Triticum aestivum defensin (TaPDF)genes,TaPDF32,TaPDF33 and TaPDF34 (GenBank accession No.BT009185,BT009167 and BT009022),were cloned from the young leaves of Jimai 22 cultivar.Sequence analysis showed that each had an open reading frame of 243,249 bp and 225 bp, which encoded the proteins with 80,82 and 74 amino acids respectively.The mature proteins,with the esti-mated molecular masses of all about 5.5 kD and the isoelectric points all higher than 7,each contained eight conservative cysteine residues.Conserved domain analysis found that TaPDF32 contained a strictly conserved gamma -thionin domain,while TaPDF33 and TaPDF34 each had a Knot1 function domain.Both of the do-mains are the typical characteristics of plant defensin.Thus,the three TaPDFs all belong to typical plant de-fensin proteins.Study of the three dimensional structure using SWISS -MODEL showed that all of them con-tained a triple -stranded anti -parallel β-sheet and an α-helix stabilized by four disulfide bridges.Phyloge-netic analysis showed that TaPDF32 and TaPDF33 were close to defensins of Zea mays and Setaria italica,while TaPDF34 was close to Clycine max defensin.But they all owned low similarities with the other reported wheat defensins,which indicated that TaPDF32,TaPDF33 and TaPDF34 were three new wheat defensins.Real -time PCR analysis revealed that TaPDF32 and TaPDF33 genes had the highest expression level in leaves,followed by seeds,and the lowest level in roots.Whereas TaPDF34 expressed basically consistent in all tissues.关键词
小麦/植物防御素/基因克隆/序列分析/组织特异性表达Key words
Triticum aestivum/Plant defensin/Gene cloning/Sequence analysis/Tissue -specific ex-pression分类
农业科技引用本文复制引用
史灵敏,张斌,丁汉凤,李娜娜,高文伟,彭振英..三个小麦防御素基因的克隆及序列分析[J].山东农业科学,2016,48(11):1-8,8.基金项目
“十二五”农村领域国家科技计划课题 （）
