山东农业科学2016，Vol.48Issue(11)：9-12,4.DOI:10.14083/j.issn.1001-4942.2016.11.002
一种玉米CRISPR／Cas9载体的构建
Construction of A Maize CRISPR/Cas9 Vector
摘要
Abstract
As its characteristics of high efficiency,easy manipulation and low cost,the clustered regular-ly interspaced short palindromic repeats (CRISPR)/CRISPR -associated (Cas)9 system has been widely used in precise modification of plant genome,which is regarded as a molecular tool with a broad application prospect.A detailed protocol of targeted mutagenesis of starch synthase regulatory factor gene ZmRSR1 in maize using the CRISPR/Cas9 system was provided,which might give a reference for site -directed modifica-tion of maize genome.关键词
CRISPR/Cas9/玉米/基因组编辑/调控因子Key words
CRISPR/Cas9/Maize/Genome editing/Regulation factor分类
农业科技引用本文复制引用
何春梅,刘铁山,关海英,刘强,董瑞,刘春晓,齐世军,贾熙,汪黎明..一种玉米CRISPR／Cas9载体的构建[J].山东农业科学,2016,48(11):9-12,4.基金项目
山东省农业科学院青年科研基金（2014QNM30）；国家转基因生物新品种培育科技重大专项（2014ZX08003003）；山东省博士后创新项目专项资金 （）
