山东农业科学2016，Vol.48Issue(11)：13-17,5.DOI:10.14083/j.issn.1001-4942.2016.11.003
野生玫瑰基因组DNA提取及CDDP引物筛选
Genomic DNA Extraction and CDDP Primer Screening of Rosa rugosa
摘要
Abstract
DNA extraction of Rosa rugosa is difficult for their high contents of polyphenol and polysac-charide.Here a modified CTAB method for Rosa rugosa DNA extraction was presented through extending water -bath time,appropriately adjusting chloroform isoamyl alcohol (24∶1)extraction time to 2 ~3 times and etc.Twenty -one CDDP polymorphic primers were screened with reaction system containing 10 μL of 2 ×Es Taq MasterMix (including dye),1 μL of 40 ng/μL DNA template,1 μL of 10 pmol /μL primer and 8 μL of ddH2 O.The results showed that the genomic DNA of Rosa rugosa extracted with modified CTAB method owned high purity,and 16 CDDP primers had higher discriminating ability on Rosa rugosa.This research pro-vided bases for CDDP molecular markers and subsequent molecular biology researches of Rosa rugosa.关键词
野生玫瑰/改良CTAB法/DNA提取/CDDP分子标记Key words
Rosa rugosa/Modified CTAB method/DNA extraction/CDDP molecular marker分类
农业科技引用本文复制引用
姜丽媛,臧德奎,李文清,解孝满,仝伯强..野生玫瑰基因组DNA提取及CDDP引物筛选[J].山东农业科学,2016,48(11):13-17,5.基金项目
山东省农业良种工程项目“林木种质资源收集保护与评价”［鲁农良字（2010）6号］ （）
