山东农业科学2016，Vol.48Issue(11)：18-20,25,4.DOI:10.14083/j.issn.1001-4942.2016.11.004
利用分子标记辅助选择培育抗稻瘟病粳稻新品系
Development of Japonica Rice Lines with Blast Resistance Genes by Molecular Marker -Assisted Selection
摘要
Abstract
Using Hua 130B as donor of rice blast resistance genes and Shengdao 15 as recipient,ten lines including four lines with Pi1 gene,three lines with Pi2 gene and three double gene -pyramided lines were developed by back -cross breeding with molecular marker -assisted selection,and these lines showed desirable agronomic traits in the field.The natural evaluation results of rice blast resistance in field showed that the disease resistance grade of single gene lines and double gene -pyramided lines were 3 and 1 respec-tively,showing high resistance.The resistance grade of Shengdao 15 was 7,so it was highly susceptible.This research indicated that breeding of Pi1 and Pi2 using molecular marker -assisted selection could significantly improve the resistance to rice blast,and it also showed that the resistance of double gene -pyramided lines was significantly better than that of single gene lines.关键词
水稻/稻瘟病/分子标记辅助选择/抗性鉴定Key words
Rice/Rice blast/Molecular marker -assisted selection/Resistance identification分类
农业科技引用本文复制引用
杨永义,张华,宣宁,陈高,柳絮,姚方印..利用分子标记辅助选择培育抗稻瘟病粳稻新品系[J].山东农业科学,2016,48(11):18-20,25,4.基金项目
公益性行业（农业）科研专项（201303007－2）；山东省现代农业产业技术体系水稻创新团队建设项目（SDAIT －17－03）；山东省农业科学院科技创新重点项目 （）
