山东农业科学2016，Vol.48Issue(11)：21-25,5.DOI:10.14083/j.issn.1001-4942.2016.11.005
无毛黄瓜同源四倍体诱导及鉴定
Induction and Identification of Autotetraploid of Glabrous Cucumber
摘要
Abstract
The germinating seeds of glabrous cucumber (2n =2x =14)were immersed in different con-centrations of mixed solution of colchicine (0.2%,0.4%,0.8%)and 2% dimethylsulfoxide (DMSO)for 4 hours.The autotetraploid (2n =4x =28)was obtained successfully by morphological and chromosome identifi-cations.The results showed that the treatment with 0.4% colchicine and 2% DMSO for 4 hours had the best induction effect with the chromosome -doubled rate of 14%.Compared with their original diploid plants,the autotetraploid plants had slower growth and development,shorter internode,thicker stem,thicker and shorter petiole,ovary and fruit,larger size of leaves and flowers,lower fruit shape index,larger leaf stomata,uneve-ner tetraploid pollen size and little quantity of quadri -aperture pollens.In addition,their chloroplast number of guard cells was almost 2 times of the original diploid plants.The results would enrich the genetic diversity and germplasm resources of cucumber,provide the theoretical basis for high quality breeding of cucumber and lay a foundation for creating euploid which was associated with the glabrous gene gl.关键词
无毛黄瓜/同源四倍体/诱导/鉴定Key words
Glabrous cucumber/Autotetraploid/Induction/Identification分类
农业科技引用本文复制引用
刘永月,许建鹏,李田田,谷停停,姜文芝,刘桂军,曹辰兴..无毛黄瓜同源四倍体诱导及鉴定[J].山东农业科学,2016,48(11):21-25,5.基金项目
2014年山东省农业良种工程项目“越冬温室黄瓜专用品种选育及开发” （）
