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好氧反硝化细菌LKX-1的分离、鉴定及初步应用研究

刘立立 兰时乐 杨友才

山东农业科学2016,Vol.48Issue(11):76-80,5.
山东农业科学2016,Vol.48Issue(11):76-80,5.DOI:10.14083/j.issn.1001-4942.2016.11.018

好氧反硝化细菌LKX-1的分离、鉴定及初步应用研究

Isolation,Identification of A Aerobic Denitrifier LKX -1 and Its Preliminary Application Research

刘立立 1兰时乐 1杨友才1

作者信息

  • 1. 湖南农业大学生物科学技术学院,湖南 长沙 410128
  • 折叠

摘要

Abstract

A high efficient aerobic denitrifier was isolated from rice field to dispose the rural domestic sewage,then the strain was identified and its nitrogen removal effect was analyzed.The results showed that the strain was identified as Pseudomonas monteilii through analyzing the cell morphology,colony morphology, physiological and biochemical characteristics combined with 16S rDNA sequence.The preliminary study on ni-trogen removal conditions of rural domestic sewage showed that with the glucose additive amount of 0.03%, inoculation amount of 0.00015%,rotation speed of 120 r/min and cultural time of 48 hours,the removal rate of TN and NO -2 -N reached 91.55% and 96.33% respectively.

关键词

好氧反硝化细菌/分离/农村生活污水/除氮率

Key words

Aerobic denitrifier/Isolation/Rural domestic sewage/Nitrogen removal rate

分类

农业科技

引用本文复制引用

刘立立,兰时乐,杨友才..好氧反硝化细菌LKX-1的分离、鉴定及初步应用研究[J].山东农业科学,2016,48(11):76-80,5.

基金项目

湖南省环保科研项目(2015-109);长沙市科技计划项目 ()

山东农业科学

OACSTPCD

1001-4942

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