中国康复理论与实践2016，Vol.22Issue(11)：1241-1245,5.DOI:10.3969/j.issn.1006-9771.2016.11.001
电针神庭、百会对脑缺血再灌注大鼠学习记忆能力和海马CA1区环磷酸腺苷效应元件结合蛋白及其磷酸化水平的影响
Effects of Electroacupuncture at Shenting and Baihui on Learning and Memory, and Expression of Cyclic AMP Response Element Binding Protein and Phosphorylation in Rats with Cerebral Ischemia-reperfusion
摘要
Abstract
Objective To explore the effects of electroacupuncture at Shenting (DU24) and Baihui (DU20) on cognitive dysfunction af-ter stroke. Methods Forty-five Sprague-Dawley rats were randomly divided into control group (n=15), model group (n=15) and electroacu-puncture group (n=15). The latter two groups were occluded their middle cerebral artery for two hours and reperfused. The electroacupunc-ture group accepted electroacupuncture at Shenting and Baihui 24 hours after modeling for seven days. They were assessed with Morris wa-ter maze once a day since the second day of intervention. Their brains were stained with TTC staining to measure cerebral infarction volume after treatment, while the expression of cyclic AMP response element binding protein (CREB) and phosphorylation (p-CREB) in hippocam-pal CA1 area were detected with immunohistochemistry. Results The escape latency and swimming distance of place navigation shortened in the electroacupuncture group compared with those in the model group (P<0.05) from the fourth day of intervention. The number of cross platform of spatial probe increased (P<0.05). The infarction volume was less in the electroacupuncture group than in the model group (P<0.05), with increased expression of CREB and p-CREB in hippocampal CA1 area (P<0.05). Conclusion Electroacupuncture at Shenting and Baihui can increase the expression of CREB and phosphorylation in hippocampal CA1 area in rats after cerebral ischemia-reperfusion, to protect the neurons from ischemia and improve the learning and memory function.关键词
脑缺血再灌注/学习记忆/电针/环磷酸腺苷效应元件结合蛋白/磷酸化/大鼠Key words
cerebral ischemia-reperfusion/learning and memory/electroacupuncture/cyclic AMP response element binding protein/phosphorylation/rats分类
医药卫生引用本文复制引用
张蕴,林如辉,李钻芳,陶静,陈立典..电针神庭、百会对脑缺血再灌注大鼠学习记忆能力和海马CA1区环磷酸腺苷效应元件结合蛋白及其磷酸化水平的影响[J].中国康复理论与实践,2016,22(11):1241-1245,5.基金项目
2016年福建省中青年教师教育科研项目(No.科技类JAT160802)。 （No.科技类JAT160802）
