中国康复理论与实践2016，Vol.22Issue(11)：1246-1251,6.DOI:10.3969/j.issn.1006-9771.2016.11.002
电针神庭、百会对脑缺血再灌注大鼠学习记忆能力和LIM激酶1磷酸化的影响
Effects of Electroacupuncture at Shenting and Baihui on Ability of Learning and Memory, and LIM-domain Con-taining Protein Kinase 1 Phosphorylation in Rats with Cerebral Ischemia-reperfusion
摘要
Abstract
Objective To investigate the effect of electroacupuncture (EA) at Shenting (DU24) and Baihui (DU20) acupoints on hippo-campal synaptic plasticity and learning and memory function, and its possible mechanism in rats with cerebral ischemia-reperfusion. Meth-ods Thirty-two male adult Sprague-Dawley rats were randomly divided into sham group (n=8), model group (n=8), electroacupuncture (EA) group (n=8) and non-acupoint group (n=8). The model group, EA group and non-acupoint group were performed with left middle cerebral artery occlusion (MCAO). The EA group received electroacupuncture at Shenting and Baihui, while the non-acupoint group received elec-troacupuncture at two fixed non-acupoints from bilateral ribs, for fourteen days. Then they were tested with Morris Water Maze test, and their synaptic structure of hippocampal neurons was observed with transmission electron microscope. The level of total LIM-domain contain-ing protein kinase 1 (T-LIMK1) and LIM-domain containing protein kinase 1 phosphorylation (p-LIMK1) were detected with Western blot-ting. Results Compared with the sham group, the latency increased (t>6.789, P<0.01) and the frequence crossing platform decreased (t=8.695, P<0.001) in the model group, while the number of synapse in hippocampal neurons decreased, and the level of T-LIMK1 (t=7.568, P<0.01) and p-LIMK1 (t=8.874, P<0.001) decreased. Compared with the model group, the latency decreased (t>4.938, P<0.01) and the fre-quence crossing platform increased (t=-7.891, P<0.001) in the EA group, while the number of synapses increased, and the level of T-LIMK1 (t=-6.473, P<0.01) and p-LIMK1 (t=-6.579, P<0.01) increased. There was no significant difference between the the non-acu-point group and the model group in all the indices (P>0.05). Conclusion Electroacupuncture at Shenting and Baihui can improve the ability of learning and memory in rats with cerebral ischemia-reperfusion, which may relate with the increase of LIMK1 phosphorylation and hippo-campal synaptic plasticity.关键词
脑缺血再灌注/电针/学习记忆/突触可塑性/LIM激酶1/磷酸化/大鼠Key words
cerebral ischemia-reperfusion/electroacupuncture/learning and memory/synaptic plasticity/LIM-domain containing pro-tein kinase 1/phosphorylation/rats分类
医药卫生引用本文复制引用
吴洁,卓沛元,林云娇,王露露,黄佳,林如辉,陶静,柳维林..电针神庭、百会对脑缺血再灌注大鼠学习记忆能力和LIM激酶1磷酸化的影响[J].中国康复理论与实践,2016,22(11):1246-1251,6.基金项目
1.国家自然科学基金项目(No.81403450)；2.福建省自然科学基金项目(No.2016J01382)。 （No.81403450）
